The AP2/EREBP family transcription factors play important roles in a wide range of stress tolerance and hormone signaling. In this study, a heat-inducible rice ERF gene was isolated and functionally characterized. The OsERF115/AP2EREBP110 was categorized to Group-IIIc of the rice AP2/EREBP family and strongly induced by heat and drought treatment. The OsERF115/AP2EREBP110 protein targeted to nuclei and suppressed the ABA-induced transcriptional activation of Rab16A promoter in rice protoplasts. Overexpression of OsERF115/AP2EREBP110 enhanced thermotolerance of seeds and vegetative growth stage plants. The OsERF115/AP2EREBP110 overexpressing (OE) plants exhibited higher proline level and increased expression of a proline biosynthesis P5CS1 gene. Phenotyping of water use dynamics of the individual plant indicates that the OsERF115/AP2EREBP110-OE plant exhibited better water saving traits under heat and drought combined stress. Our combined results suggest the potential use of OsERF115/AP2EREBP110 as a candidate gene for genetic engineering approaches to develop heat and drought stress-tolerant crops.
Percutaneous radiofrequency (RF) thermal ablation has received attention as an effective minimally invasive approach for the treatment of a variety of neoplasms. However, the therapeutic efficacy of RF thermal ablation on primary pleural synovial sarcoma has not yet been reported. A 76-year-old man with a primary pleural synovial sarcoma who was medically inoperable received RF thermal ablation to achieve local control. Therefore, an RF electrode was inserted into the lesion and connected to an RF generator. In this report, a case of successful treatment of a primary pleural synovial sarcoma using RF ablation without complication is presented.
Our study demonstrated that VSL#3 is effective in achieving clinical responses and remissions in patients with mild-to moderately active UC, further supporting the potential role in UC therapy.
Electrochemical dopamine (DA) detection has been extensively studied for the practical diagnosis of neurological disorders. A major challenge in this system is to synthesize selective and sensitive DA sensing electrocatalysts in extracellular fluids, because critical interferents such as uric acid (UA) and ascorbic acid (AA) exhibit oxidation potentials similar to those of DA. Herein, we report an extremely selective and sensitive electrocatalyst for DA sensing prepared by vanadium selenide (V2Se9). A solution-based process for the first time was introduced to synthesize the V2Se9, showing unique DA-philic characteristic caused by exposure negative charge of crystal Se. Owing to its distinctive features, the prepared V2Se9 electrode detected only DA in the presence of concentrated interferents. Electrochemical characterization and computing simulation provide strong evidence that the extreme DA selectivity stems from close physical affinity between Se and DA, leading to the outer sphere electron transfer mechanism. This is totally different from common catechol-based oxidation process. Furthermore, nano-structured V2Se9 electrode extremely improves DA sensing ability as low as practical detection limit with maintaining inactive interferent characteristic. More interestingly, an identical unique DA-sensing ability was also observed in a V2Se9 analogue—Nb2Se9. We believe that this finding provides new insights into the effect of the analyte-philic properties of electrode materials on the electrocatalytic response for selective analyte quantification.
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