The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

Park, Seong-Im; Kwon, Hyeok Jin; Cho, Mi Hyeon; Song, Ji Sun; Kim, Beom-Gi; Baek, Jeongho; Kim, Song Lim; Ji, Hyeonso; Kwon, Taek-Ryoun; Kim, Kyung-Hwan; Yoon, In Sun

doi:10.3390/ijms22137181

Cited by 24 publications

(22 citation statements)

References 97 publications

(124 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result from leaf water loss assay revealed that the water loss rate of leaves of OsRF1 -OE lines was significantly lower than that of WT after 3 to 5 h ( P < 0.05). We further investigated the water loss dynamics of OsRF1 -OE lines at whole plant level using DroughtSpotter, an automated gravimetric platform for phenotyping the drought tolerance of a plant, as described by Park et al (2021) . In this DroughtSpotter experiment, fully watered individual plants were subjected to drought stress by stopping irrigation for 1–3 days, and the weight of each pot was automatically measured every 1 min.…”

Section: Resultsmentioning

confidence: 99%

“…Water use dynamics of the WT and OsRF1-OE rice at the whole plant level was assessed using DroughtSpotter (Phenospex, Heerlen, Netherlands) as described by Park et al (2021). Briefly, a single plant was grown in a pot for 3-4 weeks and loaded on a DroughtSpotter cell located in a precisely controlled environmental room operated under a 14-h light/10-h dark photoperiod with 50% humidity between 30 • C (at noon) and 23 • C (at midnight).…”

Section: Water Loss Assaymentioning

confidence: 99%

See 1 more Smart Citation

The Rice Abscisic Acid-Responsive RING Finger E3 Ligase OsRF1 Targets OsPP2C09 for Degradation and Confers Drought and Salinity Tolerance in Rice

et al. 2022

Self Cite

View full text Add to dashboard Cite

Drought and salinity are major important factors that restrain growth and productivity of rice. In plants, many really interesting new gene (RING) finger proteins have been reported to enhance drought and salt tolerance. However, their mode of action and interacting substrates are largely unknown. Here, we identified a new small RING-H2 type E3 ligase OsRF1, which is involved in the ABA and stress responses of rice. OsRF1 transcripts were highly induced by ABA, salt, or drought treatment. Upregulation of OsRF1 in transgenic rice conferred drought and salt tolerance and increased endogenous ABA levels. Consistent with this, faster transcriptional activation of key ABA biosynthetic genes, ZEP, NCED3, and ABA4, was observed in OsRF1-OE plants compared with wild type in response to drought stress. Yeast two-hybrid assay, BiFC, and co-immunoprecipitation analysis identified clade A PP2C proteins as direct interacting partners with OsRF1. In vitro ubiquitination assay indicated that OsRF1 exhibited E3 ligase activity, and that it targeted OsPP2C09 protein for ubiquitination and degradation. Cell-free degradation assay further showed that the OsPP2C09 protein is more rapidly degraded by ABA in the OsRF1-OE rice than in the wild type. The combined results suggested that OsRF1 is a positive player of stress responses by modulating protein stability of clade A PP2C proteins, negative regulators of ABA signaling.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Water Loss Assaymentioning

confidence: 99%

The Rice Abscisic Acid-Responsive RING Finger E3 Ligase OsRF1 Targets OsPP2C09 for Degradation and Confers Drought and Salinity Tolerance in Rice

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Members of the DREB subfamily function in regulating plant resistance to abiotic stress, including drought ( Yamaguchi-Shinozakiaib and Shinozaki, 1994 ; Kole et al, 2015 ). According to Park et al (2021) genes DREB2A and DREB2B are induced by both dehydration and high-salt stress, although they do not respond to low temperature in Arabidopsis ( Park et al, 2021 ). Haake et al (2002) isolated the CBF4 gene, also named DREB1D , from Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the AP2/ERF Gene Family and Functional Analysis of GmAP2/ERF144 for Drought Tolerance in Soybean

Wang

Shen

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Drought is a major environmental constraint that causes substantial reductions in plant growth and yield. Expression of stress-related genes is largely regulated by transcription factors (TFs), including in soybean [Glycine max (L.) Merr.]. In this study, 301 GmAP2/ERF genes that encode TFs were identified in the soybean genome. The TFs were divided into five categories according to their homology. Results of previous studies were then used to select the target gene GmAP2/ERF144 from among those up-regulated by drought and salt stress in the transcriptome. According to respective tissue expression analysis and subcellular determination, the gene was highly expressed in leaves and encoded a nuclear-localized protein. To validate the function of GmAP2/ERF144, the gene was overexpressed in soybean using Agrobacterium-mediated transformation. Compared with wild-type soybean, drought resistance of overexpression lines increased significantly. Under drought treatment, leaf relative water content was significantly higher in overexpressed lines than in the wild-type genotype, whereas malondialdehyde content and electrical conductivity were significantly lower than those in the wild type. Thus, drought resistance of transgenic soybean increased with overexpression of GmAP2/ERF144. To understand overall function of the gene, network analysis was used to predict the genes that interacted with GmAP2/ERF144. Reverse-transcription quantitative PCR showed that expression of those interacting genes in two transgenic lines was 3 to 30 times higher than that in the wild type. Therefore, GmAP2/ERF144 likely interacted with those genes; however, that conclusion needs to be verified in further specific experiments.

show abstract

“…In rice, Sub1A and SNORKEL1/2 modulate flooding tolerance via two different physiological mechanisms [ 60 , 61 , 62 , 63 ]. Overexpression of OsDREB1s , OsDREB2A , OsEREBP1 , OsERF48 , OsERF71 , OsLG3 , OsERF83 , OsERF101 , OsERF115 , and OsERF4a ( OsERF3 ) improved drought tolerance in rice, through activating the jasmonate and abscisic acid (ABA) signaling pathways or modulating root architecture [ 57 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. By contrast, OsEBP89 , OsERF109 , OsDERF1 , OsERF3, and OsAP2-39 negatively regulated rice drought tolerance [ 70 , 79 , 80 , 81 , 82 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some rice OsERF genes, such as OsDREB1s , OsEREBP1 , OsSTAP1 and OsAP25 , were reported to be induced under low temperature or cold stress conditions [ 46 , 54 , 64 , 86 , 88 , 89 , 90 , 91 ], and overexpression of OsDREB1A , OsDREB1F or OsDREB1G in transgenic rice increased tolerance to cold or low-temperature stress [ 66 , 90 , 92 ]. Recently, it was found that the overexpression of OsERF115 conferred enhanced heat tolerance in transgenic rice plants [ 78 ].…”

Section: Introductionmentioning

confidence: 99%

ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

Hong

Wang

Gao

et al. 2022

IJMS

View full text Add to dashboard Cite

We previously showed that overexpression of the rice ERF transcription factor gene OsBIERF3 in tobacco increased resistance against different pathogens. Here, we report the function of OsBIERF3 in rice immunity and abiotic stress tolerance. Expression of OsBIERF3 was induced by Xanthomonas oryzae pv. oryzae, hormones (e.g., salicylic acid, methyl jasmonate, 1-aminocyclopropane-1-carboxylic acid, and abscisic acid), and abiotic stress (e.g., drought, salt and cold stress). OsBIERF3 has transcriptional activation activity that depends on its C-terminal region. The OsBIERF3-overexpressing (OsBIERF3-OE) plants exhibited increased resistance while OsBIERF3-suppressed (OsBIERF3-Ri) plants displayed decreased resistance to Magnaporthe oryzae and X. oryzae pv. oryzae. A set of genes including those for PRs and MAPK kinases were up-regulated in OsBIERF3-OE plants. Cell wall biosynthetic enzyme genes were up-regulated in OsBIERF3-OE plants but down-regulated in OsBIERF3-Ri plants; accordingly, cell walls became thicker in OsBIERF3-OE plants but thinner in OsBIERF3-Ri plants than WT plants. The OsBIERF3-OE plants attenuated while OsBIERF3-Ri plants enhanced cold tolerance, accompanied by altered expression of cold-responsive genes and proline accumulation. Exogenous abscisic acid and 1-aminocyclopropane-1-carboxylic acid, a precursor of ethylene biosynthesis, restored the attenuated cold tolerance in OsBIERF3-OE plants while exogenous AgNO3, an inhibitor of ethylene action, significantly suppressed the enhanced cold tolerance in OsBIERF3-Ri plants. These data demonstrate that OsBIERF3 positively contributes to immunity against M. oryzae and X. oryzae pv. oryzae but negatively regulates cold stress tolerance in rice.

show abstract

The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants

Cited by 24 publications

References 97 publications

The Rice Abscisic Acid-Responsive RING Finger E3 Ligase OsRF1 Targets OsPP2C09 for Degradation and Confers Drought and Salinity Tolerance in Rice

The Rice Abscisic Acid-Responsive RING Finger E3 Ligase OsRF1 Targets OsPP2C09 for Degradation and Confers Drought and Salinity Tolerance in Rice

Genome-Wide Identification of the AP2/ERF Gene Family and Functional Analysis of GmAP2/ERF144 for Drought Tolerance in Soybean

ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

Contact Info

Product

Resources

About