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Introduction: Pain is considered an unpleasant sensory and emotional experience, being considered as one of the most important causes of human suffering. Computational chemistry associated with bioinformatics has stood out in the process of developing new drugs, through natural products, to manage this condition. Objective: To analyze, through literature data, recent molecular coupling studies on the antinociceptive activity of essential oils and monoterpenes. Data source: Systematic search of the literature considering the years of publications between 2005 and December 2019, in the electronic databases PubMed and Science Direct. Eligibility Criteria: Were considered as criteria of 1) Biological activity: non-clinical effects of an OE and/or monoterpenes on antinociceptive activity based on animal models and in silico analysis, 2) studies with plant material: chemically characterized essential oils and/or their constituents isolated, 3) clinical and non-clinical studies with in silico analysis to assess antinociceptive activity, 4) articles published in English. Exclusion criteria were literature review, report or case series, meta-analysis, theses, dissertations, and book chapter. Results: Of 16,006 articles, 16 articles fulfilled all the criteria. All selected studies were non-clinical. The most prominent plant families used were Asteraceae, Euphorbiaceae, Verbenaceae, Lamiaceae, and Lauraceae. Among the phytochemicals studied were a-Terpineol, 3-(5-substituted-1,3,4-oxadiazol-2-yl)-N′-[2-oxo-1,2-dihydro-3H-indol-3ylidene] propane hydrazide, b-cyclodextrin complexed with citronellal, (−)-a-bisabolol, b-cyclodextrin complexed with farnesol, and p-Cymene. The softwares used for docking studies were Molegro Virtual Docker, Sybyl ® X, Vlife MDS, AutoDock Vina, Hex Protein Docking, and AutoDock 4.2 in PyRx 0.9. The molecular targets/complexes used were Nitric Oxide Synthase, COX-2, GluR2-S1S2, TRPV1, b-CD complex, CaV 1 , CaV 2.1 , CaV 2.2 , and CaV 2.3 , 5-HT receptor, delta receptor, kappa receptor, and MU (m)

2-Allylphenol (2-AP) is a synthetic phenylpropanoid, structurally related to cardanol, thymol, and ortho-eugenol. Phenylpropanoids are described in the literature as being capable of promoting biological activity. Due to the similarity between 2-AP and other bioactive phenylpropanoids, the present research aims at evaluating the antioxidant, antinociceptive, and anti-inflammatory potential of 2-AP in silico, in vitro, and in vivo. At 30 min prior to the start of in vivo pharmacological testing, administration of 2-AP (25, 50, 75, and 100 mg/kg i.p.), morphine (6 mg/kg i.p.), dexamethasone (2 mg/kg s.c.), or vehicle alone was performed. In the acetic acid-induced abdominal writhing tests, pretreatment with 2-AP significantly reduced the number of abdominal writhes, as well as decreased licking times in the glutamate and formalin tests. Investigation of the mechanism of action using the formalin model led to the conclusion that the opioid system does not participate in its activity. However, the adenosinergic system is involved. In the peritonitis tests, 2-AP inhibited leukocyte migration and reduced releases of proinflammatory mediators TNF-α and IL-1β. In vitro antioxidant assays demonstrated that 2-AP presents significant ability to sequester superoxide radicals. In silico docking studies confirmed interaction between 2-AP and the adenosine A2a receptor through hydrogen bonds with the critical asparagine 253 residues present in the active site. Investigation of 2-AP demonstrated its nociception inhibition and ability to reduce reactive oxygen species. Its interaction with A2a receptors may well be related to proinflammatory cytokines TNF-α and IL-1β reduction activity, corroborating its antinociceptive effect.

A infecção do trato urinário (ITU) é uma das mais frequentes que acometem os pacientes internos em ambiente hospitalar, podendo ser classificada como cistite ou pielonefrite, de acordo com o sítio da infecção. O Diagnóstico da ITU é feito basicamente pela realização do sumário de urina (EAS) e da urocultura. Sendo assim, o objetivo deste trabalho foi realizar estudo retrospectivo no período de janeiro/2021 a dezembro/2021 com base no banco de dados do laboratório de análises clínicas do Hospital Universitário Lauro Wanderley (HULW) no estado da Paraíba em relação às alterações apresentadas no exame de EAS, principalmente com os marcadores nitrito e esterase leucocitária de uroculturas positivas. Também foi observado os dados epidemiológicos dessas infecções, assim como a incidência bacteriana das mesmas. Obteve-se um total de 1817 exames de Uroculturas realizados, sendo que 837 exames apresentavam solicitação de EAS concomitantemente, e destas 129 amostras de urocultura foram positivas sendo os microrganismos prevalente as Escherichia coli (36,5%), Klebsiella pneumoniae (20,6%) e Pseudomonas aeruginosa (8,7%). O nitrito foi observado em 16% das amostras e a esterase leucocitária em 58%. Concluímos que quanto aos resultados do EAS, a leucocitúria revelou-se um marcador mais sensível para auxílio diagnóstico de ITU em relação ao nitrito, corroborando com a literatura científica especializada, com predomínio de ITU em pacientes do sexo feminino.

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Warifteine therapeutic treatment reduced leukocyte recruitment and anxietylike response in ovalbumin-induced allergic pulmonary inflammationTratamento terapêutico com warifteína reduz recrutamento de leucócitos e resposta semelhante á ansiedade na inflamação pulmonar alérgica induzida por ovalbumina

O eritrócito é um tipo celular altamente suscetível peroxidação lipídica e hemólise. Testes in vitro de citotoxicidade são frequentemente usados ​​para rastrear e determinar a toxicidade de vários compostos, principalmente para investigar efeitos diretos sobre a integridade da membrana. As cumarinas (1,2-benzopirona) fazem parte de um grupo de compostos heterocíclicos presentes em várias famílias de plantas. Inúmeras atividades biológicas têm sido demonstradas para cumarinas e seus derivados, incluindo propriedades anti-inflamatórias, antioxidantes, anticancerígenas e antimicrobianas. O objetivo do presente estudo foi investigar pela primeira vez o perfil tóxico de um derivado cumarínico, o ácido 3-cumarino carboxílico, em ensaios citotoxicidade envolvendo eritrócitos humanos. Foram preparadas soluções contendo o ácido 3-cumarino carboxílico nas concentrações de 50, 100, 500 e 1000 µg/mL. Amostras de sangue humano do tipo A, B e O foram coletadas de voluntários saudáveis e submetidas à avaliação de citotoxicidade frente aos ensaios de atividade hemolítica e anti-hemolítica. A substância testada foi capaz de reduzir a lise sobre os eritrócitos humanos dos tipos sanguíneos A, B e O em todas as concentrações testadas. No ensaio de fragilidade osmótica o ácido 3-cumarino carboxílico também foi capaz de proteger os eritrócitos humanos contra a hemólise, nos tipos sanguíneos A, B e O, nas concentrações de 50µg/mL e 100µg/mL. Os resultados de citotoxicidade in vitro indicam que o ácido 3-cumarino carboxílico apresentou baixo percentual de hemólise para eritrócitos humanos dos grupos sanguíneos A, B e O quando em contato direto com essas células, sendo também capaz de proteger a membrana eritrocitária, impedindo a hemólise.

The objective of this work was to analyze the hematological parameters of patients positive for COVID-19, admitted to the COVID intensive care unit (ICU-COVID) of the University Hospital Lauro Wanderley (HULW), to correlate these data with the clinical conditions of critically ill patients, compared to patients in the ward of the COVID Infectious Parasitic Diseases Unit (IDP -COVID). The study population consisted of patients in (ICU-COVID) and (IDP-COVID) of both genders, considering children, adolescents, adults, and elderly people hospitalized in this unit with positive serology or molecular tests (RT-PCR). This was a retrospective observational cross-sectional study involving patients in the (ICU-COVID) and (IDP -COVID) of the HULW. The study was carried out in accordance with the requirements of the Research Ethics Committee (CAAE 46718921.4.0000.5183) of the HULW. 152 exams were analyzed. Of these, 88 (58%) were male and the average in relation to the age group was 61.5 years. By correlating the data obtained from inpatients in the COVID-ICU and IDP-COVID, it can be observed in the ICU-COVID patients there was the predominance of leukocytosis (50.4%), as well as lymphopenia (90.9%), neutrophilia (80%) and anemia (70%). Thrombocytopenia is present in 23% of patients in the COVID-ICU and alterations in Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) were identified. These data corroborate the literature, in which the total leukocyte count varies between patients, which may reflect the dominance of lymphopenia or neutrophilia. Taken together, decreased lymphocytes accompanied by mild thrombocytopenia are among the most common abnormal findings that attract attention in the blood count of COVID-19 patients.