This article describes a new method for analysis of IR fingerprint spectra (FPS), which characterizes traditional Chinese medicine (TCM). Two new indices of IR FPS are introduced, namely, common peak ratio and variation peak ratio. Their values are calculated by means of the sequent analysis method, in which each sample's IR FPS of the components extracted from Glycyrrhizae root is as a standard, and the sequences are made in accordance with the values of their
proteomics is able to reveal plentiful information related to different physiological and pathological states of biology. Further, the determination of accurately proteomic pattern is the essential platform for deeply proteomic research. While this has been somewhat ignored so far. In this article the quantitative standard Pg=61%, a biological similarity constant for discriminating accurately intrinsic proteomic patterns was established depending on biological common heredity and variation information equation in symmetric variation state. On the other hand, a novel theoretical method was proposed for linearly dividing nonlinear data sequence into linear segments. The proteomes of three kind soybeans were precisely distinguished from one another by analyzing their infrared fingerprint spectra relying on this theoretically systemic approach. Additionally, methods employed in this paper enable us to quickly, accurately and quantitatively determine the proteomic patterns without using any prior knowledge and learning samples, and without using electrophoresis, high performance liquid chromatography-mass spectrometry techniques, which are high cost, time-consuming. This approach provide us with an excellent one for quickly accurate determining biological species, physiological states and diagnosing pathological states based on proteomes.
The enhanced luminescence of the Eudibenzoylmethane(DBM) -NH3system in the presence ofTb3+ and the effect of solvents on that system in the absence of Tb3+ were studied. The optimum conditions for co-luminescence of the Eu -Tb -DBM -NH3 system were examined. The optimised procedure was applied to the determination of trace amounts of Eu. The detection limit is 4.0 x 10-11 M, which is about two orders of magnitude lower than that of the system in the absence of Tb. An intermolecular transfer of energy from the enhancing complex to the fluorescing complex is proposed.
To overcome the instability of traditional Chinese medicine (TCM) fingerprint spectra (FPS) and to build up absolute identification standard of TCM, the construction method of combinational numeral fingerprint spectra (CNFPS) was set up. The analysis of invariableness based on CNFPS was carried out. It can be used as absolute quantitative standard to identify the difference of TCM samples. According to this method, the HPLC FPS of components extracted from Glycyrrhiza root samples by ethanol was analyzed. Stable results and accurate quality evaluation were obtained perfectly. The common peak ratio invariableness is an absolute standard in identifying TCM samples, which is better than the similarity method of the FPS.
As traditional Chinese medicine (TCM) is gradually accepted by many countries, people pay much attention to the quality of herbal medicines. Because of the significant variation in active components in them, the quality control of herbal medicines is a very important issue. Nowadays, high-performance liquid chromatography (HPLC) fingerprint spectra (FPS) are widely used in identification and quality control of herbal medicines. This paper will analyze the methodology and their application in identifying and evaluating herbal medicines by means of HPLC FPS, which includes simple comparing, clustering, principal component analysis (PCA), and similarity analysis methods.
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