Combinational numeral fingerprint spectra of Glycyrrhiza and analysis of common peak ratio invariableness in HPLC

Abstract

To overcome the instability of traditional Chinese medicine (TCM) fingerprint spectra (FPS) and to build up absolute identification standard of TCM, the construction method of combinational numeral fingerprint spectra (CNFPS) was set up. The analysis of invariableness based on CNFPS was carried out. It can be used as absolute quantitative standard to identify the difference of TCM samples. According to this method, the HPLC FPS of components extracted from Glycyrrhiza root samples by ethanol was analyzed. Stable results and accurate quality evaluation were obtained perfectly. The common peak ratio invariableness is an absolute standard in identifying TCM samples, which is better than the similarity method of the FPS.

Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by high-performance liquid chromatography

“…One is the qualitative and quantitative analysis of one or several high-content components. The other is analysis of chemical fingerprint [13], which has been introduced and accepted by the WHO [14], FDA (2000) [15] and other authorities as a strategy for quality assessment of herbal medicines. In the past decade, the chromatographic fingerprints established by HPLC, HPTLC, GC, and CE have been recognized as rapid and reliable means for the identification and qualification of herbal medicines [16,17].…”

Quality Control Methodology and Their Application in Analysis on HPLC Fingerprint Spectra of Herbal Medicines

“…Zou et al [38] proposed an approach named as the combinational numeral fingerprint spectra invariableness method, with which Glycyrrhiza herbal medicines can be classified based on the absolute parameter-invariableness. This method calculates the invariableness between inner group samples and inter group samples of the same kind, which is able to represent the characteristic properties of one kind of sample and is chosen as the absolute coefficient belonging to this kind of samples.…”

Two Chinese medicine species constants and the accurate identification of Chinese medicines

“…Author Zou has paid a great effort to searching for the mathematical theory for identifying traditional Chinese medicines and biological systems for long time, grounded on intrinsic properties and elemental principle in nature. He proposed and established independently the common and variant peak ratio dual index sequence analysis method during his doctorate work [7][8][9][10][11][12][13]. This theory has been adopted by many researchers to identify herbs,plants, foods and animals, etc, and more than 50 papers using this method have been published so far,such as papers [14][15][16][17][18][19][20][21][22][23][24][25][26].…”