Assumption of a water-impermeable coat on bacterial spores is inconsistent with known permeabilities of organic materials. A low water content may arise through compressive contraction of the cortex during spore maturation.
California Small White beans were found to contain about 1% phytate, of which 70% is in water-soluble form. Incubation of presoaked beans in water at 60°C for 10 hr lowered their phytate content by 90%. Incubation in pH 5.5 buffer at 50°C for 24 hr lowered it by 62%. When leaching was eliminated by incubating in water-saturated air at 60°C overnight, 33% of the phytate was destroyed by hydrolysis. Similar treatments were effective with mung beans, lima beans, and wheat. The study suggests that destruction of heat-sensitive celI membranes leads to the potentiation of phytase.
Thesis submitted by . K. Burr to the University of Wisconsin in partial fulfillment of the requirements for the degree of Doctor of Philosophy, September, 1941.
A series of successive chemical and physical fractionations of cooked dry California Small White beans were made, and the activity of each fraction was measured, using human subjects, for its ability to increase the carbon dioxide component of flatus. The activity was found to be: extractable with 60 % aqueous ethanol; dialyzable through a reconstituted cellulose membrane; soluble in 85% ethanol; and unabsorbed by a column packed with a strong cation exchange resin. This active column fraction was shown to contain the sugars, fructose; sucrose, raffinose, stachyose, and at least four polypeptides hydrolyzable into 22 amino acids. Raffinose and stachyose fed alone at levels found in California Small White beans did not increase the carbon dioxide level of the flatus.
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