Besides inciting persistent and recurrent nosocomial afflictions, Staphylococcus aureus (S. aureus), a biofilm forming pathogen, poses an increased risk of several skin as well as respiratory tract infections as well. Emerging antimicrobial resistance trend asks to search for an alternate non-antibiotic based option to combat S. aureus pathogen. In the present study, we evaluated synergistic antimicrobial potential of Zinc oxide nanorods (ZnO-NRs) and diallyl sulphide (DAS) emulsion against methicillin resistant Staphylococcus aureus (MRSA). The antimicrobial assessment study suggests that the ZnO-NR and DAS emulsion effectively suppressed both sensitive S. aureus as well as MRSA isolates. The combination treatment showed enhanced activity even at a lower concentration as compared to the single treatment based on ZnO-NRs and DAS emulsion alone. The ZnO-NRs-DAS combination showed significant inhibition of MRSA biofilm as well. The data suggest that a combination therapy, comprising of ZnO-NRs and DAS emulsion, successfully treated experimental dermatitis infection caused by MRSA in mice model.
Starch [(C6H10O5)
n
]-stabilized bismuth sulfide (Bi2S3) nanoparticles (NPs) were synthesized in a single-pot
reaction
using bismuth nitrate pentahydrate (Bi(NO3)3·5H2O) and sodium sulfide (Na2S) as precursors.
Bi2S3 NPs were stable over time and a wide band
gap of 2.86 eV was observed. The capping of starch on the Bi2S3 NPs prevents them from agglomeration and provides regular
uniform shapes. The synthesized Bi2S3 NPs were
quasispherical, and the measured average particle size was ∼11
nm. The NPs are crystalline with an orthorhombic structure as determined
by powder X-ray diffraction and transmission electron microscopy.
The existence and interaction of starch on the NP’s surface
were analyzed using circular dichroism. Impedance spectroscopy was
used to measure the electronic behavior of Bi2S3 NPs at various temperatures and frequencies. The dielectric measurements
on the NPs show high dielectric polarizations. Furthermore, it was
observed that the synthesized Bi2S3 NPs inhibited
bacterial strains (Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus
aureus) and demonstrated substantial antibacterial
activity.
Objective: Determining whether non-surgical periodontal therapy (NSPT) will affect bone turnover biomarkers (BTMs) concentration in individuals with periodontitis.
Materials and Methods: 60 females aged 18-45 years were divided into two groups Normal (N) (n= 20) and Chronic periodontitis (CP) (n= 40) after recruitment. Participants with periodontitis were assigned (NSPT). Full mouth clinical periodontal parameters, salivary and serum BTMs including C-terminal telopeptides of type I collagen (CTX), bone alkaline phosphatase (BALP), and Osteoprotegerin (OPG) were recorded for both groups and 21 days and 3 months after NSPT.
Results: Except for BALP_ SER (p<0.023), all variables had highly significant differences (p<0.001). Except for CP (21 days) vs. CP (3 months), groups showed significantly greater levels of PI, PPD and CAL. Correlations between BTMs and periodontal measurements were weak to moderate after NSPT. The multiple linear regression model explained 46.7% of the total variability for PI, 50.1% for PPD, 54.1% for CAL and 29.3% for GI signifying association with BTMs among groups
Conclusion: Our research showed the concentration of BTMs is greatly impacted by NSPT demonstrating the significance of NSPT on bone health in periodontitis. Additionally, we discovered that serum instead of salivary samples can be utilized as a diagnostic tool to measure BTMs concentration.
Clinical Relevance- This study emphasizes the significance of regular periodontal evaluation and the use of non-surgical periodontal therapy (NSPT) to manage periodontitis and prevent further bone loss. It also highlights the value of using serum samples as a diagnostic tool to monitor bone health in periodontitis patients.
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