Synergistic Effect of Diallyl Sulfide With Zinc Oxide Nanorods: A Novel and Effective Approach for Treatment of Acute Dermatitis in Model Animals

Rauf, Mohd Ahmar; Zubair, Swaleha; Ateeq, Hira; Dabeer, Khadija; Pachauri, Subodh; Ajmal, Mohd; Owais, Mohammad

doi:10.3389/fmicb.2018.00586

Cited by 19 publications

(9 citation statements)

References 57 publications

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“…The biofilm assay was performed according to the X.T.T. protocol, which has been recently reported by our laboratory [20,35]. Briefly, the fresh biofilms formation in the plate (96 wells) was eroded with the PBS to take out the non-attached bacterial cells.…”

Section: Anti-biofilm Activitymentioning

confidence: 99%

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Graphene Decorated Zinc Oxide and Curcumin to Disinfect the Methicillin-Resistant Staphylococcus aureus

Oves

Rauf

Ansari³

et al. 2020

Nanomaterials

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Sometimes, life-threatening infections are initiated by the biofilm formation facilitated at the infection site by the drug-resistant bacteria Staphylococcus aureus. The aggregation of the same type of bacteria leads to biofilm formation on the delicate tissue, dental plaque, and skin. In the present investigation, a Graphene (Gr)-based nano-formulation containing Curcumin (C.C.M.) and Zinc oxide nanoparticles (ZnO-NPs) showed a wide range of anti-microbial activity against Methicillin-resistant Staphylococcus aureus (MRSA) biofilm and demonstrated the anti-microbial mechanism of action. The anti-microbial effect of GrZnO nanocomposites, i.e., GrZnO-NCs, suggests that the integrated graphene-based nanocomposites effectively suppressed both sensitive as well as MRSA ATCC 43300 and BAA-1708 isolates. The S. aureus inhibitory effect of GrZnO-NCs improved >5-fold when combined with C.C.M., and demonstrated a M.I.C. of 31.25 µg/mL contrasting with the GrZnO-NCs or C.C.M. alone having M.I.C. value of 125 µg/mL each. The combination treatment of GrZnO-NCs or C.C.M. inhibited the M.R.S.A. topical dermatitis infection in a mice model with a significant decrease in the CFU count to ~64%. Interestingly, the combination of C.C.M. and GrZnO-NCs damaged the bacterial cell wall structure, resulting in cytoplasm spillage, thereby diminishing their metabolism. Thus, owing to the ease of synthesis and highly efficient anti-microbial properties, the present graphene-based curcumin nano-formulations can cater to a new treatment methodology against M.R.S.A.

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Section: Anti-biofilm Activitymentioning

confidence: 99%

“…can correspond with the modulation of virulence, quorum sensing, as well as biofilm inhibition, etc. [20][21][22]. The previous reports have demonstrated the antibacterial capability of C.C.M.…”

Section: Introductionmentioning

confidence: 95%

Graphene Decorated Zinc Oxide and Curcumin to Disinfect the Methicillin-Resistant Staphylococcus aureus

Oves

Rauf

Ansari³

et al. 2020

Nanomaterials

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“…2 . A broad band at 3200–3435 cm −1 can be attributed to stretching H-bound water, and 1410–1622 cm −1 can be ascribed to the bending vibrations of the hydroxide (–OH) groups absorbed at the surface of samples 48 . The weak bands observed at 2364, 1095 and 1041 cm −1 were related to the vibrations of the organic residuals.…”

Section: Resultsmentioning

confidence: 99%

Facile one-pot green synthesis of Ag–ZnO Nanocomposites using potato peeland their Ag concentration dependent photocatalytic properties

Alharthi

Alghamdi

Al‐Zaqri

et al. 2020

Sci Rep

128

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Herein, a facile green synthesis route was reported for the synthesis of Ag–ZnO nanocomposites using potato residue by simple and cost effective combustion route and investigated the photocatalytic degradation of methylene blue (MB) dye. In the preparation potato extract functioned as a biogenic reducing as well as stabilizing agent for the reduction of Ag + , thus eliminating the need for conventional reducing/stabilizing agents. Ag–ZnO nanocomposites with different Ag mass fractions ranging from 2 to 10% were characterized by using XRD, FT-IR, XPS, SEM, TEM, and UV–Vis spectroscopy. XRD analysis revealed that the as prepared Ag–ZnO nanocomposites possessed high crystallinity with hexagonal wurtzite structure. TEM and SEM images showed that the Ag–ZnO nanocomposites in size ranging from 15 to 25 nm have been obtained, and the particle size was found to increase with the increase in percentage of Ag. FTIR results confirmed the characteristics band of ZnO along with the Ag bands. XPS analysis revealed a pair of doublet with peaks corresponding to Ag and a singlet with peaks corresponding to ZnO. With the increase of concentration of Ag in ZnO, the intensity of NBE emission in the PL spectra was observed to be decrease, resulted to the high photocatalytic activity. Photocatalytic properties of Ag–ZnO nanocomposites evaluated against the MB dye under visible-light irradiation showed superior photodegradation of ~ 96% within 80 min for 2% Ag–ZnO nanocomposites. The apparent reaction rate constant for 2% Ag–ZnO nanocomposites was higher than that of other nanocomposites, which proved to be the best photocatalyst for the maximum degradation of MB. Furthermore, various functional parameters such as dosing, reaction medium, concentration variation were performed on it for better understanding. The enhancement in photocatalytic degradation might be due to the presence of Ag nanoparticles on the surface of ZnO by minimizing the recombination of photo induced charge carriers in the nanocomposites.

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“…Turbidity was checked as an indicator of the growth of the bacterial organism in the presence of AgNPs. The least amount dose of AgNP that inhibited the growth of the organism indicated the minimum inhibitory concentration (Rauf et al, 2018).…”

Section: Materials Methodsmentioning

confidence: 99%

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

et al. 2019

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Considering the significance of biological and eco-friendly nanomaterials, in the present study, we have synthesized silver nanoparticles from the exopolysaccharide of recently recovered bacterial strain CEES51 from the Red Sea coastal area of Jeddah, Saudi Arabia. 16S ribosomal RNA gene sequencing was used to characterize the isolated bacteria, and it was identified as Mesoflavibacter zeaxanthinifaciens and assigned an accession number MH707257.1 GenBank. The bacterial strain is an excellent exopolysaccharide producer and survived at hypersaline (30%) and high-temperature (50°C) conditions. The bacterial exopolysaccharides were employed for the fabrication of silver nanoparticles at room temperature. UV-visible spectrophotometer optimized the synthesized nanoparticles, and their size was determined by Nanophox particle size analyzer and dynamic light scattering. Additionally, the X-ray powder diffraction and Fourier-transform infrared spectroscopy studies also approved its crystalline nature and the involvement of organic functional groups in their formation. The synthesized nanomaterials were tested for their antibacterial and antibiofilm properties against pathogenic microorganisms Bacillus subtilis and methicillin-resistant Staphylococcus aureus . The antimicrobial property showed time, and dose-dependent response with a maximum of zone inhibition was observed at around 22 and 18 mm at a dose of 50 µg/well against B. subtilis and S. aureus and a minimum inhibitory concentration of 8 and 10 µg/ml, respectively. Furthermore, the synthesized silver nanoparticles possessed a substantial antibiofilm property and were also found to be biocompatible as depicted by red blood cell lysis assay and their interaction with peripheral blood mononuclear cells and human embryonic kidney 293 cells. Therefore, Mesoflavibacter zeaxanthinifaciens is found to be an excellent source for exopolysaccharide synthesis that assists in the silver nanoparticle production.

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Synergistic Effect of Diallyl Sulfide With Zinc Oxide Nanorods: A Novel and Effective Approach for Treatment of Acute Dermatitis in Model Animals

Cited by 19 publications

References 57 publications

Graphene Decorated Zinc Oxide and Curcumin to Disinfect the Methicillin-Resistant Staphylococcus aureus

Graphene Decorated Zinc Oxide and Curcumin to Disinfect the Methicillin-Resistant Staphylococcus aureus

Facile one-pot green synthesis of Ag–ZnO Nanocomposites using potato peeland their Ag concentration dependent photocatalytic properties

Antibacterial Silver Nanomaterial Synthesis From Mesoflavibacter zeaxanthinifaciens and Targeting Biofilm Formation

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