Rhodamine B is a textile coloring materials which often mixed in food coloring. The use of Rhodamine B in food for a long time may result in liver dysfunction or cancer. However, when exposed Rhodamine B in large quantities in a short time it will be symptoms of acute poisoning of Rhodamine B. The allicin and alliin content in garlic works as an antioxidant that can neutralize free radicals there by lowering oxidative stress and help prevent an increase in SGOT and SGPT and improve depiction hepatic histopathology. The purpose of this study are to determine the effect of preventive therapy extract water of garlic (Allium sativum) on levels of SGOT and the SGPT and liver histopathology description of rat (Rattus norvegicus) which were exposed by Rhodamine B. This study used male rats strain Wistar with 8 weeks of age and weight of 200 grams which were divided into 5 groups: group A (negative control), B (positive control), group C, D, and E were fed with rodhamin B and given preventive water with garlic juice with successive doses of 0.5 mL, 1 mL, and 1.5 mL. SGPT and SGOT level measurements performed by spectrophotometric method and observation of rat liver histopathology performed using light microscope. The data analysis activities of SGPT/SGOT using ANOVA, and the description of histopathology were analyzed descriptively. The results showed giving garlic extract with a dose of 1.5 mL/0.2 kg bw were able to decrease the activity of SGPT, i.e. respectively for treatments A to E, 18.89%, 17.3 %, 40.96% and SGOT 41.44%, 45.96%, 49.69%, and to improve the hepatocyte cells in rat exposed to Rhodamine B. The conclusion of this study is water garlic juice can be used as herbal therapy in mice which were exposed by Rhodamine B.
Penelitian ini mengkaji gaya bahasa yang terdapat pada postingan akun instagram @kumpulan_puisi . Adapun tujuan penelitian ini yaitu mendeskripsikan jenis gaya bahasa yang terdapat pada postingan akun instagram @kumpulan_puisi. Metode yang digunakan dalam penelitian ini adalah metode kualitatif yang bersifat deskriptif. Sumber data dalam penelitian ini yaitu postingan akun instagram @kumpulan_puisi periode Agustus-Desember 2021 yang mengandung jenis gaya bahasa. Adapun data penelitian ini adalah kata atau kalimat pada postingan akun instagram @kumpulan_puisi periode Agustus-Desember 2021 yang mengandung jenis gaya bahasa. Teknik pengumpulan data yaitu studi pustaka, dokumentasi, simak dan catat. Teori yang digunakan yaitu teori yang dimukakan oleh Tarigan mengenai jenis-jenis gaya bahasa, meliputi gaya bahasa perbandingan, pertentangan, dan perulangan. Berdasarkan hasil penelitian ditemukan 33 data gaya bahasa dengan rincian gaya bahasa perbandingan 13 data, terdiri dari 7 buah data gaya bahasa perumpamaan, 4 buah data gaya bahasa metafora, dan 2 buah data gaya bahasa personifikasi. Selanjutnya, terdapat Gaya bahasa pertentangan berjumlah 7 data, terdiri dari 4 buah data gaya bahasa hiperbola, dan 3 buah data gaya bahasa ironi, dan yang terakhir ditemukan 13 data Gaya bahasa perulangan, terdiri dari 2 buah gaya bahasa aliterasi, 9 buah data gaya bahasa anafora, dan 2 buah data gaya bahasa Epizeukis.Kata kunci: Stilistika, majas, akun instagram
Dioxin is one of the environment's persistent organic pollutants that harm animal and human health. Prolonged exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can disrupt normal organ function including heart. Yogurt is known to have antioxidant properties, and fortification natural plant-based antioxidant purple roselle (Hibiscus sabdariffa L) extract into yogurt can improve the antioxidant potential. The purpose of this study was to determine the preventive effects of yogurt fortified with purple roselle on dioxin exposure rats (Rattus norvegicus) based on the levels of Malondialdehyde (MDA) and histopathological of the heart. This experimental study used a completely randomized design (CRD). A total of 30 male Wistar rats were divided into five groups (Negative Control, Positive Control, Treatment groups T1, T2, and T3), a dose of 200 ng.kg -1 BW TCDD and 1 mL of roselle yogurt 0.5%, 1.0%, and 1.5% were given orally for 12 days. MDA levels were analyzed using the Thiobarbituric Acid (TBA) assay and histopathology of the heart using the Haematoxylin Eosin (HE) staining method. Statistical analysis used one-way Analysis of Variance (ANOVA). Histopathology changes were analyzed descriptively. The results showed that TCDD exposure induced oxidative stress in heart tissue, and yogurt roselle has the potential to prevent an increase of MDA levels significantly (p<0.05). Furthermore, TCDD can cause histopathological alterations, such as necrosis and hemorrhage, and yogurt roselle was proven to prevent cardiotoxicity due to TCDD exposure.
Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.
Trypanosomiasis merupakan penyakit yang disebabkan oleh protozoa darah ekstraseluler berflagela yaitu Trypanosoma sp. Trypanosoma lewisi adalah parasit darah pada tikus, yang ditularkan oleh pinjal Xenopsylla cheopis. Walaupun parasit ini bersifat non patogen, tetapi keberadaannya dapat mengancam kesehatan manusia. Kasus infeksi T. lewisi pada manusia telah dilaporkan di Thailand dan India, yang mengindikasikan bahwa penyakit ini dapat menginfeksi manusia dalam beberapa keadaan yang belum diketahui. Penelitian ini bertujuan untuk mengidentifikasi T. lewisi berdasarkan morfologi dan morfometrik serta dilakukan perhitungan prevalensi dan pemetaan spasial kasus infeksi T. lewisi pada tikus liar di Malang. Sebanyak 74 ekor tikus dikumpulkan dari berbagai wilayah di Malang dengan menggunakan perangkap hidup tunggal. Hasil penelitian menunjukkan prevalensi infeksi T. lewisi pada tikus liar di Malang dari bulan Agustus sampai Oktober 2020 sebesar 17,5%. Analisis data menggunakan uji chi-square dan uji Fisher, diperoleh hasil tidak ada hubungan atau korelasi yang signifikan antara infeksi T. lewisi dengan jenis kelamin dan umur tikus. Morfologi T. lewisi memiliki posterior tipis dengan kinetoplas oval di sub-terminal, dan nukleus di anterior. Secara morfometrik, T. lewisi memiliki panjang rata-rata 33,19 μm, lebar 3,52 μm, panjang inti 7,82 μm, lebar inti 3,05 μm, panjang kinetoplas 5,25 μm, serta jarak inti ke kinetoplas 10,79 μm. Dari hasil di atas dapat disimpulkan bahwa Trypanosoma lewisi menginfeksi tikus liar dan hasil gambaran geografis didapatkan bahwa terdapat risiko penyebaran penyakit trypanosomiasis di wilayah Malang.
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