Resumo -A soja é a espécie leguminosa mais importante mundialmente e por isso investimentos em tecnologia são necessários para o aumento da produtividade e redução de fatores adversos à cultura. Na região Sul do Brasil, são frequentes os problemas ocasionados pelo acamamento de plantas de algumas cultivares de soja. Este trabalho objetivou avaliar os efeitos da aplicação de herbicidas inibidores da enzima Protoporfirinogênio Oxidase (PROTOX) sobre o desenvolvimento, rendimento de grãos e componentes e sobre o acamamento das plantas do cultivar de soja CD 214 RR. O experimento foi conduzido a campo, em delineamento blocos ao acaso, com quatro repetições, em esquema bifatorial. O fator A foi constituído por cinco tratamentos herbicidas: carfentrazone nas doses 6, 10 e 22 g ha -1 de i.a., lactofen 144 g ha -1 de i.a. e testemunha sem aplicação. O fator B foi constituído por seis épocas de avaliação de injúria e sete épocas para altura de planta e acamamento. Foram determinadas a injúria, altura, acamamento, componentes e rendimento de grãos de soja e rendimento biológico aparente. Os herbicidas lactofen e carfentrazone reduziram a altura e, consequentemente, o acamamento das plantas de soja. Carfentrazone foi mais fitotóxico e mais eficaz em reduzir a altura de plantas de soja do que lactofen. Lactofen resultou em rendimento de grãos de soja mais elevados do que as diferentes concentrações de carfentrazone, embora ambos não tenham diferido do rendimento de grãos da testemunha sem herbicidas. Palavras-chaves: carfentrazone-ethyl; Glycine max; lactofen Abstract -Soybean is the most important leguminous species worldwide and therefore investments in technologies are needed to increase productivity and reduce adverse factors to the crop. In Southern Brazil, there are often problems caused by lodging of plants from some soybean cultivars. This study aimed to evaluate the effects of application of Protoporfirinogênio Oxidase 1 Recebido para publicação em

ABSTRACT To study the efficiency of Asian soybean rust (Phakopsora pachyrhizi) control and to test the effects of using alternative products and fungicides, two field experiments were conducted during the harvest seasons 2013/2014 and 2014/2015. Treatments for the experiments were: 1- control (water); 2- acibenzolar-S-methyl; 3- calcium; 4- micronutrients: copper, manganese and zinc; 5- micronutrients: manganese, zinc and molybdenum; 6- NK fertilizer; 7- Ascophyllum nodosum, and 8- azoxystrobin + cyproconazole. The evaluated variables were: Asian soybean rust severity, defoliation, productivity and yield components. Based on severity data, the area under the disease progress curve was calculated. In the two experiments, the alternative products had no effect on Asian soybean rust or defoliation. Only the treatment with fungicide controlled the disease and decreased defoliation. The yield component affected by the treatments was the number of pods per plant, in both harvest seasons. Treatment with the fungicide protected soybean from yield loss in both experiments. Defoliation was strongly correlated with the weight of one thousand seeds and the number of pods per plant, emphasizing the importance of maintaining the leaf area to ensure the maintenance of the yield components in soybean crop.

Trichoderma asperellum e Bacillus subtilis como antagonistas no crescimento de fungos fitopatogênicos in vitroTrichoderma asperellum and Bacillus subtilis as antagonists in the growth of phytopathogenic fungi in vitro

Plum (Prunus salicina Lindl.) cultivation is seriously compromised in Brazil due to the occurrence of leaf scald, a disease caused by the bacterium Xylella fastidiosa subsp. multiplex. The objective of this study was to evaluate plum genotypes agronomically promising for resistance to leaf scald, under natural conditions of occurrence of the disease. Ten out of 50 plum genotypes belonging to the germplasm bank of “Instituto Agronômico do Paraná (IAPAR)” were selected. Five evaluations of leaf scald severity were carried out from January to February (2017 and 2018). Polymerase chain reaction (PCR) was also performed for samples of these genotypes to determine the presence of the bacterium X. fastidiosa subsp. multiplex. The genotypes differed in relation to their susceptibility to leaf scald. PR-1095 was the most resistant genotype and did not show any leaf symptom of the disease. In contrast, genotypes PR-1126 and PR-1137 had the greatest susceptibility to the disease, showing severe scald symptoms. However, PCR revealed the presence of the bacterium even in the most resistant genotypes, indicating, for example, that PR-1095, which had no symptoms of the disease, is probably tolerant to leaf scald.

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The occurrence of powdery mildew (Microsphaera diffusa) in soybean (Glycine max L.) has increased in the last harvests. In order to study the efficiency of powdery mildew control due to the application of alternative products and conventional fungicide, trials were conducted in Ponta Grossa, PR, Brazil, during the 2013/2014 and 2014/2015 growing seasons. The design used was randomized blocks with four replications. The treatments for the experiments were: 1 - control; 2 - acibenzolar-S-methyl (Bion 500 WG®); 3 - calcium (Max Fruit®); 4 - Micronutrients: copper, manganese and zinc (Wert Plus®); 5 - Micronutrients: manganese, zinc and molybdenum (V6®); 6 - NK fertilizer (Hight Roots®); 7 - Ascophyllum nodosum (Acadian®) and 8 - fungicide (azoxystrobin + cyproconazole) (Priori XTRA®) with the addition of the adjuvant. Four applications of alternative products (phenological stages V3, V6, R1 and R5.1) and two of fungicide (phenological stages R1 and R5.1) were carried out. The parameters evaluated were powdery mildew severity and productivity. The severity data made it possible to calculate the area under the disease progress curve (AUDPG). Alternative products didn’t reduce powdery mildew in the two harvests. The conventional fungicide treatment was the only one that controlled powdery mildew and didn’t reduce the productivity in both experiments.

The very slow-growing gram-negative bacterium, Xylella fastidiosa, inhabits the xylem and is vector-borne. It causes several diseases in plants, including plum leaf scald (PLS), which is the main limiting factor over the years for the crop expansion, both in productivity and in cultivation areas in Brazil. There is little information about the management and control of the disease. Brazilian breeding programs of plum trees have launched few resistant cultivars. The aim of this work was to carry out a systematic review with current information regarding PLS, characteristics of the causative agent, symptoms, transmission/dissemination, control and prevention. The use of certified propagation material, free from bacteria and the elimination of infected plants are the main measures used in Brazil. The vast majority of commercial cultivars are highly susceptible to bacteria and only few cultivars of interest have any resistance. In 2017 the Agricultural Research and Rural Extension Company of Santa Catarina launched the ‘Zafira’ plum cultivar, the first cultivar for commercial purposes that is not naturally infected; however, it was observed that the transmission of the bacteria occurs by grafting. The Institute of Rural Development of Paraná IAPAR-EMATER evaluated different plum crosses and concluded that the ‘PR-1095’ genotype was the most resistant and did not show any foliar symptoms of the disease. However, the PCR test revealed the presence of the bacteria, indicating that the genotype is probably tolerant. In contrast, ‘PR 1142’, ‘PR 1149’ and ‘PR 1260’ genotypes do not show symptoms or the presence of the bacteria by PCR, resulting in resistance to the disease. These genotypes have not been released, and further studies are still needed.

Soybean mildew caused by Oomycota Peronospora manshurica, is a disease widely spread in Brazil. In order to study the efficiency of soybean mildew control due to the application of alternative products and fungicide in the field, experiments were conducted in Ponta Grossa, PR, Brazil, during the 2013/2014 and 2014/2015 growing seasons. The design used was randomized blocks with four replications. The treatments were: 1-witness; 2-acibenzolar-S-methyl; 3-calcium; 4-micronutrients: copper, manganese and zinc; 5-micronutrients: manganese, zinc and molybdenum; 6-NK fertilizer; 7-Ascophyllum nodosum and 8-azoxystrobin + cyproconazole with the addition of Nimbus adjuvant. Four applications of alternative products (phenological stages V3, V6, R1 and R5.1) and two of fungicide (phenological stages R1 and R5.1) were performed. The mildew severity was estimated using a diagrammatic scale. The severity data made it possible to calculate the area under the disease progress curve (AUDPC). In the 2014/2015 harvest the disease was more severe. The control of downy mildew by the use of fungicide did not reduce the epidemic. The fungicide was not efficient in the two evaluated seasons. All tested alternative products reduced the disease severity and AUDPC in both seasons. The best results in reducing downy mildew were found with the application of acibenzolar-S-methyl, micronutrients (Cu, Mn, Zn) and A. nodosum.