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An opportunistic bacterial infection preceded by weather of unusually high humidity and temperature caused mass death of saigas.

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This study used gross and histological examination to highlight the large variety of naturally occurring gastric lesions in a mixed population of horses. Analysis of the pathogenesis of lesion development is now possible. Further research regarding the range of pathology in larger, more diverse groups of horses is required.

Background Haematopoietic stem cells expressing the CD34 surface marker have been posited as a niche for Mycobacterium tuberculosis complex bacilli during latent tuberculosis infection. Our aim was to determine whether M tuberculosis complex DNA is detectable in CD34-positive peripheral blood mononuclear cells (PBMCs) isolated from asymptomatic adults living in a setting with a high tuberculosis burden.Methods We did a cross-sectional study in Ethiopia between Nov 22, 2017, and Jan 10, 2019. Digital PCR (dPCR) was used to determine whether M tuberculosis complex DNA was detectable in PBMCs isolated from 100 mL blood taken from asymptomatic adults with HIV infection or a history of recent household or occupational exposure to an index case of human or bovine tuberculosis. Participants were recruited from HIV clinics, tuberculosis clinics, and cattle farms in and around Addis Ababa. A nested prospective study was done in a subset of HIV-infected individuals to evaluate whether administration of isoniazid preventive therapy was effective in clearing M tuberculosis complex DNA from PBMCs. Follow-up was done between July 20, 2018, and Feb 13, 2019. QuantiFERON-TB Gold assays were also done on all baseline and follow-up samples. Findings Valid dPCR data (ie, droplet counts >10 000 per well) were available for paired CD34-positive and CD34-negative PBMC fractions from 197 (70%) of 284 participants who contributed data to cross-sectional analyses. M tuberculosis complex DNA was detected in PBMCs of 156 of 197 participants with valid dPCR data (79%, 95% CI 74-85). It was more commonly present in CD34-positive than in CD34-negative fractions (154 [73%] of 197 vs 46 [23%] of 197; p<0•0001). Prevalence of dPCR-detected M tuberculosis complex DNA did not differ between QuantiFERONnegative and QuantiFERON-positive participants (77 [78%] of 99 vs 79 [81%] of 98; p=0•73), but it was higher in HIV-infected than in HIV-uninfected participants (67 [89%] of 75 vs 89 [73%] of 122, p=0•0065). By contrast, the proportion of QuantiFERON-positive participants was lower in HIV-infected than in HIV-uninfected participants (25 [33%] of 75 vs 73 [60%] of 122; p<0•0001). Administration of isoniazid preventive therapy reduced the prevalence of dPCR-detected M tuberculosis complex DNA from 41 (95%) of 43 HIV-infected individuals at baseline to 23 (53%) of 43 after treatment (p<0•0001), but it did not affect the prevalence of QuantiFERON positivity (17 [40%] of 43 at baseline vs 13 [30%] of 43 after treatment; p=0•13). Interpretation We report a novel molecular microbiological biomarker of latent tuberculosis infection with properties that are distinct from those of a commercial interferon-γ release assay. Our findings implicate the bone marrow as a niche for M tuberculosis in latently infected individuals. Detection of M tuberculosis complex DNA in PBMCs has potential applications in the diagnosis of latent tuberculosis infection, in monitoring response to preventive therapy, and as an outcome measure in clinical trials of interventions to prev...

This case report describes an unusual presentation of histiocytic sarcoma in a domestic shorthair cat. Initial investigation revealed a haemodynamically insignificant hypertrophic cardiomyopathy, bronchitis and a mild irregularity of the cervical trachea. The cat's disease progressed over a two-week period. Repeat radiography and tracheoscopy revealed a marked dynamic tracheal collapse associated with a raised plaque-like lesion within the cervical trachea. Subsequent post-mortem examination and histopathology revealed disseminated histiocytic sarcoma involving the trachea and kidneys. This is the first reported case of a histiocytic sarcoma involving the trachea in either dogs or cats.

Ovine pulmonary adenocarcinoma is a chronic respiratory disease of sheep caused by jaagsiekte sheep retrovirus (JSRV). OPA is a significant economic problem for sheep farmers in many countries and is a valuable animal model for some forms of human lung cancer. Here, we examined the changes in host gene expression that occur in the lung in response to JSRV infection. We identified a large number of genes with altered expression in infected lung, including factors with roles in cancer and immune system function. We also compared the data from OPA to previously published data from human lung adenocarcinoma and found a large degree of overlap in the genes that were dysregulated. The results of this study provide exciting new avenues for future studies of OPA and may have comparative relevance for understanding human lung cancer.

SummaryThe montane cloud-forests of the north-central Andes and the montane grassland and transitional elfin forest of the central Andean paramo contain a high diversity of bird species including several restricted range and uncommon species. Little is known of how densities of Andean cloud-forest species are affected by habitat degradation. Bird densities within pristine and degraded habitats at the Guandera Biological Reserve, Carchi province, Ecuador were recorded over a 10-week period. Densities were calculated for 48 species; where densities could be compared, 69% of species occurred at a higher density in pristine habitats. Pristine forest had the highest species richness with 72 species and paramo contained 44 species. In total, 26% of pristine forest species were only found in pristine forest, 39% of paramo species only in paramo, 13% of farmland species only in farmland and there were no exclusively secondary scrub species; 47% of species found in pristine forest, and 50% found in paramo were found in both secondary scrub and farmland. Restricted range species recorded at Guandera included the Carujiculated Caracara Phalcobenus carunculatus, Black-thighed Puffleg Eriocnemis derbyi, Chestnut-bellied Cotinga Doliornis remseni, Crescent-faced Antpitta Grallaricula lineifrons, Masked Mountain-tanager Buthraupis wetmorei and Black-backed Bush-tanager Urothraupis stolzmanni. Three further species that occurred at Guandera of relatively local occurrence were the Grey-breasted Mountain Toucan Andigena hypoglauca, Golden-breasted Puffleg Eriocnemis mosquera and Mountain Avocetbill Opisthoprora euryptera. Of these nine species at least five used degraded habitats, while three occurred only in pristine treeline habitats.