The aim of this research is to study the formation of acrylamide with different kind of vegetable oils as the cooking media. The samples were prepared by cooking and baking above 120℃ then extracted with dichloromethane-ethanol and separated by SPE (C-18) with methanol 60% as eluent. The extracts were analysed by HPLC, with condition as followed: C-18 column; acetonitrile-water (5:95) pH 2.52 mobile-phase; 0.5 ml/minute flow rate; and 210 nm wavelength. It was figured out that a fried tempe using corn oil contained 0.5778 μg/g acrylamide (8.202.10-3 standard deviation and 1.4195% coefficient variation), using coconut oil 0.192 μg/g acrylamide (5.656.10-3 standard deviation 2.946% coefficient variation), using palm oil 0.1455 μg/g acrylamide (6.081.10-3 standard deviation and 4.1794% coefficient variation).
Ginseng Jawa (Talinum triangular) merupakan tanaman yang seluruh bagian tanamannya memiliki khasiat sebagai obat. Tujuan penelitian ini adalah optimalisasi zat pengatur tumbuh untuk inisasi kalus ginseng jawa. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Kebun Raya Cibodas – Badan Riset dan Inovasi Nasional (BRIN) pada bulan Agustus sampai bulan November 2021. Metode penelitian yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan sembilan kombinasi perlakuan 2,4-D (Dichlorophenoxyatic Acid) and Benzyl Adenine (BA) dengan tiga ulangan. Eksplan daun berdiameter 8 mm ditanam pada botol kultur berisi media Murashige and Skoog yang mengandung kombinasi konsentrasi zat pengatur tumbuh 2,4-D (0,5 ppm, 1 ppm, dan 1,5 ppm) dan BA (0,5 ppm, 1 ppm, dan 1,5 ppm). Eksplan yang ditanaman mampu menghasilkan kalus dengan waktu awal muncul kalus antara 13-22 hari setelah inisiasi dan memiliki tekstur remah pada semua perlakuan. Terdapat empat warna kalus yang dihasilkan dari perlakuan ini, yaitu moderate yellow green, grayish yellow green, pale olive dan pale brown. Terdapat kombinasi konsentrasi terbaik diperoleh pada perlakuan D2B3 (1 ppm 2,4-D + 1,5 ppm BA) yang memberikan pertumbuhan optimal pada parameter panjang kalus sebesar 3,17 cm dan parameter berat basah kalus sebesar 2,16 gram serta berat kering kalus sebesar 0,21 gram.
Email: hayatulrahmi.@staff.unsika.ac.id ABSTRAK Antioksidan adalah senyawa yang dapat menangkap aktivitas radikal bebas karena bisa menyumbangkan satu elektronnya pada radikal bebas. Tujuan penelitian ini adalah untuk mengetahui seberapa besar nilai aktivitas antioksidan ekstrak daun dan kulit buah kawista asal Kabupaten Karawang. Penelitian ini merupakan penelitian eksperimental, metode penelitian menggunakan cara ekstraksi (maserasi) dengan pelarut aquades dingin, aquades panas, dan etanol 96%. Pengujian aktivitas antioksidan dapat dilakukan dengan pengukuran sarapan radikal DPPH (1,1-difenil-2-pikrilhidrazil) menggunakan Spektrofotometer UV-Vis. Hasil penelitian yaitu diperoleh Nilai aktivitas antioksidan ekstrak daun kawista tertinggi dengan pelarut air panas yaitu 134,56 ppm dan aktivitas antioksidan tertinggi pada sampel kulit buah kawista dicapai oleh pelarut etanol dengan nilai 381,36 ppm.
Kata kunci : antioksidan, UV-Vis, ekstrak daun dan kulit buah kawista
ABSTRACTAntioxidants are compounds that can capture free radical activity because they can donate one electron to free radicals. The purpose of this study was to determine how much the antioxidant activity of leaf extract and kawista fruit skin from Karawang Regency. This research is an experimental, the research method used extraction (maceration) with cold aquades, hot aquades, and ethanol 96%. Tested of antioxidant activity can be done by measuring DPPH radical breakfast (1,1-diphenyl-2-picrylhydrazyl) using a UV-Vis spectrophotometer. The results were obtained by the highest antioxidant activity of kawista leaf extract with hot water solvents : 134.56 ppm and the highest antioxidant activity in kawista fruit skin samples achieved by ethanol solvents with a value of 381.36 ppm..
Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
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