The Activity of Wungu Leaf (&lt;i&gt;Graptophyllum pictum&lt;/i&gt; (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice

Rahmi, Hayatul; Artika, I Made; Azwar, Norman Razief; Seno, Djarot Sasongko Hami; Nurcholis, Waras

doi:10.29244/cb.1.2.83-88

Cited by 3 publications

(2 citation statements)

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“…G. pictum administered via the oral route effectively reduced blood glucose levels in alloxan-induced diabetes in mice at doses between 50 mg/kg and 200 mg/kg [ 54 ]. G. pictum extracts administered orally at 50 mg/kg over a period of 28 days were shown to decrease the glucose levels in blood by percentages ranging from 30 to 37% [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Silico Evaluation of Anticholinesterase and Antidiabetic Effects of Furanolabdanes and Other Constituents from Graptophyllum pictum (Linn.) Griffith

Metiefeng,

Tamfu,

Fotsing Tagatsing

et al. 2023

Molecules

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Graptophyllum pictum is a tropical plant noticeable for its variegated leaves and exploited for various medicinal purposes. In this study, seven compounds, including three furanolabdane diterpenoids, i.e., Hypopurin E, Hypopurin A and Hypopurin B, as well as with Lupeol, β-sitosterol 3-O-β-d-glucopyranoside, stigmasterol 3-O-β-d-glucopyranoside and a mixture of β-sitosterol and stigmasterol, were isolated from G. pictum, and their structures were deduced from ESI-TOF-MS, HR-ESI-TOF-MS, 1D and 2D NMR experiments. The compounds were evaluated for their anticholinesterase activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BchE), as well as their antidiabetic potential through inhibition of α-glucosidase and α-amylase. For AChE inhibition, no sample had IC50 within tested concentrations, though the most potent was Hypopurin A, which had a percentage inhibition of 40.18 ± 0.75%, compared to 85.91 ± 0.58% for galantamine, at 100 µg/mL. BChE was more susceptible to the leaves extract (IC50 = 58.21 ± 0.65 µg/mL), stem extract (IC50 = 67.05 ± 0.82 µg/mL), Hypopurin A (IC50 = 58.00 ± 0.90 µg/mL), Hypopurin B (IC50 = 67.05 ± 0.92 µg/mL) and Hypopurin E (IC50 = 86.90 ± 0.76 µg/mL). In the antidiabetic assay, the furanolabdane diterpenoids, lupeol and the extracts had moderate to good activities. Against α-glucosidase, lupeol, Hypopurin E, Hypopurin A and Hypopurin B had appreciable activities but the leaves (IC50 = 48.90 ± 0.17 µg/mL) and stem (IC50 = 45.61 ± 0.56 µg/mL) extracts were more active than the pure compounds. In the α-amylase assay, stem extract (IC50 = 64.47 ± 0.78 µg/mL), Hypopurin A (IC50 = 60.68 ± 0.55 µg/mL) and Hypopurin B (IC50 = 69.51 ± 1.30 µg/mL) had moderate activities compared to the standard acarbose (IC50 = 32.25 ± 0.36 µg/mL). Molecular docking was performed to determine the binding modes and free binding energies of Hypopurin E, Hypopurin A and Hypopurin B in relation to the enzymes and decipher the structure–activity relationship. The results indicated that G. pictum and its compounds could, in general, be used in the development of therapies for Alzheimer’s disease and diabetes.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Silico Evaluation of Anticholinesterase and Antidiabetic Effects of Furanolabdanes and Other Constituents from Graptophyllum pictum (Linn.) Griffith

Metiefeng,

Tamfu,

Fotsing Tagatsing

et al. 2023

Molecules

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“…The result revealed that all extracts decreased blood glucose level with a percentage of decrease ranging from 30 to 37% compared to glibenclamide (0.25 mg/kg) with a value of 56%. 19 Estrogenic effects 24 Oral treatment with hexane leaf extract of G. pictum (0.5 mg/0.05 ml olive oil) possessed an estrogenic effect by significantly increasing the diameter of the uterus, mucous layer thickness, lumen epithelial cell height, and glandular epithelial cell height compared to aquadesttreated group. However, the estrogenic effect of G. pictum remains significantly lower than Ethinyl estradiol as the positive control.…”

Section: Anti-diabetic Effectmentioning

confidence: 99%

Graptophyllum pictum (L.) Griff. (Syn: Justicia picta Linn.) and its Effectiveness: A Well-Known Indonesian Plant

Makkiyah¹,

Rahmi²,

Revina³

et al. 2021

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Graptophyllum pictum (L.) Griff., a shrubby stove plant, has been used traditionally to treat various diseases. This review summarizes the up-to-date data about its phytochemistry and pharmacological properties and provides a perspective and direction for future research. The phytochemical analysis of G. pictum revealed the presence of flavonoids, steroids, glycoside, tannins, saponin, chlorophyll, nontoxic alkaloid, and anthocyanin. Meanwhile, the pharmacological analysis revealed that this plant possessed various activities such as antimicrobial, immunomodulatory, antioxidant, anti-inflammation, anti-analgetic, woundhealing, anti-hemorrhoid, anti-diabetic, and estrogenic effects. However, most of its pharmacological activities were based on the crude extracts and the bioactive compounds responsible for the bioactivities have not been well identified. A further detailed investigation in phytochemistry to identify the bioactive compounds and to investigate the mechanism action of its bioactivities is required. Moreover, a toxicology study is also needed to develop a standardized formulation of G. pictum preparation and to fulfill the requirements of modern phytomedicine.

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Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae

2020

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Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern. The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae. Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol. The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067. Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%. In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%. Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.

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The Activity of Wungu Leaf (<i>Graptophyllum pictum</i> (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice

Cited by 3 publications

References 2 publications

In Vitro and In Silico Evaluation of Anticholinesterase and Antidiabetic Effects of Furanolabdanes and Other Constituents from Graptophyllum pictum (Linn.) Griffith

In Vitro and In Silico Evaluation of Anticholinesterase and Antidiabetic Effects of Furanolabdanes and Other Constituents from Graptophyllum pictum (Linn.) Griffith

Graptophyllum pictum (L.) Griff. (Syn: Justicia picta Linn.) and its Effectiveness: A Well-Known Indonesian Plant

Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae

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