<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle">This research aimed to obtain the performance of super intensive white shrimp rearing using zero water discharge (ZWD) system. This study consisted of four steps, (1) activation and cultivation of nitrifying bacteria, microalgae <em>C. calcitrans</em> and <em>B. megaterium</em>; (2) acclimatization of Pacific white shrimp PL10 with 30 g/L of salinity and decreasing salinity at 2–3 g/L/day; (3) conditioning of ZWD system; (4) white shrimp rearing in 400 L of tank for ten weeks. The experiment used three treatments, (a) shrimp reared without any addition of microbial agent with water exchange conducted every week as much as10–20% of total rearing volume as control (K); (b) ZWD systems with the applications of nitrifying bacteria, (<em>C. calcitrans</em> and <em>B. megaterium</em>) without water discharge (P1); and (c) ZWD system with the application of microalgae <em>C. calcitrans</em> and <em>B. megaterum</em> without water discharge (P2). According to the results, application of nitrifying bacteria, microalgae <em>C. calcitrans</em> and <em>B. megaterium</em> were able to improve the performance of ZWD system performance of white shrimp rearing at low salinity. In addition, the ZWD system was also able to increase the growth rate and survival rate of shrimp when it compared to control. The best rearing performance was found in ZWD system with application of microalgae <em>C. calcitrans</em> and <em>B. megaterium</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Litopenaeus vannamei</em>, ZWD, low salinity, microalgae, nitrification bacteria.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mengetahui kinerja budidaya udang putih super intensif bersalinitas rendah menggunakan sistem <em>zero water discharge</em> (ZWD). Penelitian ini terbagi dalam tiga, yaitu (1) aktivasi dan kultur bakteri nitrifikasi, mikroalga <em>C. calcitrans</em> dan <em>B. megaterium</em>; (2) aklimatisasi udang putih PL10 salinitas 30 g/L dan penurunan salinitas 2–3 g/L/hari; (3) pengondisian dari sistem ZWD; (4) pemeliharaan udang putih selama 10 minggu di bak bervolume 400 L. Penelitian ini menggunakan tiga perlakuan ; (a) perlakuan kontrol tanpa penambahan mikroba dan pergantian air setiap minggu sebanyak 10–20% (K) ; (b) sistem ZWD dengan bakteri nitrifikasi, mikroalga <em>C. calcitrans</em> dan <em>B. megaterium</em> tanpa pergantian air (P1); (c) sistem ZWD dengan mikroalga <em>C. calcitrans </em>dan <em>B. megaterum </em>tanpa pergantian air (P2). Berdasarkan hasil yang didapat, aplikasi bakteri nitrifikasi, mikroalga C<em>. calcitrans</em> dan <em>B. megaterum</em> mampu meningkatkan kineja sistem ZWD pada budidaya udang putih <em>L. vannamei </em>bersalinitas rendah. Selain itu, aplikasi bakteri nitrifikasi, mikroalga <em>C. calcitrans</em> dan <em>B. megaterum </em>pada sistem ZWD juga mampu meningkatkan laju pertumbuhan dan sintasan udang putih dibanding dengan kontrol. Kinerja pemeliharaan terbaik dijumpai pada sistem ZWD dengan aplikasi mikroalga <em>C. calcitrans</em> dan <em>B. megaterum</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: bakteri nitrifikasi, <em>Litopenaeus vannamei</em>, mikroalga, salinitas rendah, ZWD </p>
The high concentration of detergent in the aquatic ecosystem potentially affects the physiological condition of the fish by disrupting the respiration process and changing the concentration of blood components and chemistry. The purpose of this study was to determine the condition of the hematological parameters of tilapia (Oreochromis niloticus) exposed to wastewater from the laundry industry. Each treatment was stocked with five fish per aquarium (50 x 30 x 30 cm). This study used a completely randomized design (CRD) technique with six treatments and three replications: P0 (0%) as a control, P1 (1%), P2 (2%), P3 (3%), P4 (4%) and P5 (5%) with each treatment exposed to a specific concentration of wastewater and residues. The results showed that the hemoglobin levels of treatments were decreasing, with the lowest mean of hemoglobin level found in the P2 (7.05 gr%), and the lowest concentration on the 30th day was 7.71 gr%. There were no significant effects of wastewater to the number of erythrocytes and leucocytes among treatments (P>0.05). While there were increasing levels of hematocrit, the largest mean level was found in the P4 treatment with a value of 24.11%, and the largest mean on the 20th day of observation showed a value of 23.51%. Wastewater from the laundry industry can affect the hematological condition of tilapia by decreasing the hemoglobin concentration and increasing the hematocrit levels above the normal condition.
Biosurfactant production by Pseudomonas aeruginosa by utilizing diesel as the source of energy and carbon was studied in this research. Biosurfactant-producing strain of the organism was isolated from hydrocarbon-contaminated water and grown in a mineral medium supplemented with diesel. Production of biosurfactant was assayed by monitoring the increase in cell concentration, biosurfactant concentration, emulsification index and decrease in surface tension. Highest level of cell concentration and biosurfactant concentration (3.3^10 8 CFU/ml and 0.0108mg/ml respectively) were obtained at 132hrs. Emulsification index reached its maximum with 33.3% at 108hrs. Surface tension reached its minimum at 120hrs with a value of 1.1^10 -6 N/m. The results showed that diesel can be utilized by P. aeruginosa to produce biosurfactant and early stationary phase isolates can be used to obtain higher yield. It also revealed the increasing potentiality of microorganisms in the aspect of oil spill cleanup and rapid reclamation of contaminated lands and water bodies.
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