The medicinal plant Delphenium brunonianum collected from Manang district, Nepal has been selected for its study on ethnomedicinal uses, phytochemical investigation and bioactivity evaluation. This plant is found to be used extensively by the community for the treatment of fever, headache, stomachache and poison removal. From phytochemical investigation, four known compounds namely ?-amyrin (1), ?-sitosterol (2), ?-sitosterol glucoside (3) and anthriscifoldine (4) have been isolated from methanol extract of D. brunonianum. Compound 1 is triterpene, 2 and 3 are sterols, whereas, 4 is a diterpenoid alkaloid. The structures of all compounds were determined with modern spectroscopic techniques including 1D- and 2D- Nuclear Magnetic Resonance techniques and comparison with literature data. The extract and all compounds exhibited antibacterial properties with respect to the zone of inhibition and minimum inhibitory concentration against Bacillus subtilis, staphylococcus aureus, Pseudomonas aureginous, Escherchia coli and Salmonella flexinarie. Compound 4 was found to be more active than other compounds, and even than the standards ampicillin and gentamicin in most of the tested bacteria.DOI: http://dx.doi.org/10.3126/njst.v12i0.6488 Nepal Journal of Science and Technology 12 (2011) 111-118
The study on nutritional value of locally preferred fodder trees in the farmland of middle hills of Nepal was conducted in Hemja VDC of Kaski district. Primary information on distribution and frequency of fodder trees was obtained through key informants survey, group discussion and observation of the study area. The preference ranking of ten most abundant fodder trees was done on the basis of palatability, propagation easiness, growth rate and competition with agricultural crops. The nutritional value of fodder species was analyzed and compared with the farmers' preference ranking to examine association among them. The analysis correspond farmers' preference of fodder tree species to their nutritional values. The study revealed that Ficus subinisa was the dominant fodder tree however, the Artocarpus lakoocha was highly preferred trees for its palatability and nourishing values. Nutritional analysis of ten preferred fodder species with respect to moisture, ash, crude protein, crude fat, crude fiber and carbohydrate was carried out. The crude protein varied from 15-29%, in which, A. lakoocha to contained the highest amount of crude protein. Similarly, F. lacor contained highest crude fiber (42.07%), and Machilus odoratissima yielded highest amount of carbohydrate (21.92%).
The multipurpose Himalayan mushroom Morchella conica is one of the most popular wild high valued edible mushrooms throughout the world. It makes a good income generating source for rural livelihood. This study mainly focuses on the nutritional constituents of M. conica and its medicinal uses with social and financial values for rural livelihood. Nutritional analysis was conducted based on moisture, ash, crude fats, proteins and carbohydrates. The highest value of carbohydrate was 36.5% followed by protein 35.0%, crude fiber 28.8%, crude fat 12.0%, ash 8.2% and moisture 8.0%. In social and financial aspects of M. conica in the studied area, 20% ethnic groups involved for collection of this species. M. conica is economically high priced in which 60.0% poor and 53.3% medium class people are considering this to support their livelihood. The study also showed that this species is a potential source of diet and income for rural people.DOI: http://dx.doi.org/10.3126/njst.v12i0.6489 Nepal Journal of Science and Technology 12 (2011) 119-126
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