An Assessment of Ethnomedicinal Use, Chemical Constituents Analysis and Bioactivity Evaluation on High Altitude Medicinal Plant Delphinium brunonianum of Manang District

Abstract

The medicinal plant Delphenium brunonianum collected from Manang district, Nepal has been selected for its study on ethnomedicinal uses, phytochemical investigation and bioactivity evaluation. This plant is found to be used extensively by the community for the treatment of fever, headache, stomachache and poison removal. From phytochemical investigation, four known compounds namely ?-amyrin (1), ?-sitosterol (2), ?-sitosterol glucoside (3) and anthriscifoldine (4) have been isolated from methanol extract of D. brunonianum. Compound 1 is triterpene, 2 and 3 are sterols, whereas, 4 is a diterpenoid alkaloid. The structures of all compounds were determined with modern spectroscopic techniques including 1D- and 2D- Nuclear Magnetic Resonance techniques and comparison with literature data. The extract and all compounds exhibited antibacterial properties with respect to the zone of inhibition and minimum inhibitory concentration against Bacillus subtilis, staphylococcus aureus, Pseudomonas aureginous, Escherchia coli and Salmonella flexinarie. Compound 4 was found to be more active than other compounds, and even than the standards ampicillin and gentamicin in most of the tested bacteria.DOI: http://dx.doi.org/10.3126/njst.v12i0.6488 Nepal Journal of Science and Technology 12 (2011) 111-118

Triterpenoids and Steroid from the Leaves of Callistemon citrinus (Curtis.)

“…The above spectral features are in close agreement to those observed for β-sitosterol. 9,10 Besides, a doublet at δ 4.14 (J = 6.8 Hz) integrated for one proton indicated the presence of an anomeric proton (H-1') of a glucoside moiety. The large coupling constant (6.8 Hz) of this proton demonstrated the beta (β) linkage between the aglycone and glycone moieties.…”

“…All of these data are in close agreement with the βsitosterol-3-O-β-D-glucoside. 10 Thus, compound 3 was characterized as β-sitosterol-3-O-β-D-glucoside. Its identity was also confirmed by co-TLC with authentic sample.…”

Human Poisoning due to Delphinium species in the Himalayan Region of Nepal: A Case Report

“…exhibited antibacterial properties against various bacteria like Sstaphylococcus aureus, Pseudomonas aureginous, Escherchia coli, etc. 2 However, other extracts like methylsuccimidoanthronyllycoctonine (MSAL) and the lycoctonine group of alkaloids found in Delphinium peregrinum have the toxic effect of causing neuromuscular paralysis via competitive inhibition of post-synaptic neurotransmitter acetylcholine, specifically acting at the α1 nicotinic sites in the brain and muscle, causing respiratory depression and other curare-like symptoms. 4 , 5 Other Delphinium alkaloids like methyllycaconitine (MLA), nudicauline, barbinine, deltalineetc also block neuromuscular transmission by acting as nicotinic receptor antagonist.…”

“…1 Their roots have been used for the treatment of fever, headache, stomachache, jaundice, and skin rashes. 2 However, some species are toxic and the toxicity of the genus Delphiniumis variable depending on growth stages and concentration of a toxic substance. 3 Poisoning usually occurs in grazing cattle and is rare in humans.…”

Efficacy of rtsa-byugs vs diclofenac gel in relieving knee pain of patients with osteoarthritis of the knee

“…Apart from its anti-inflammation capacity, a study by Tripathee [20] reported that the Delphinium brunoniaum extract, which is mixed in rtsa-byugs, exhibits antibacterial properties. For example, the ability to inhibit bacillus subtilis, salmonella flexinarie, pseudomonas aureginous and staphylococcus aureus.…”