27The current research work is focused on screening of antimicrobial, antioxidant, total phenolic, 28 total flavonoid, metal estimation and proximate potential of four parts (fruits, leaves, stem bark 29 and root bark) of S. xanthocarpum. Antimicrobial potential of dried crude extracts of S. 30 xanthocarpum were evaluated against two gram positive bacteria (S. aureus, P. vulgaris), three 31 gram negative bacteria (P. aeruginosa, K. pneumonia, E. coli), three fungi (A. flavus, F. solani, 32 R. stolonifer) and two yeasts (S. cerevisiae, C. albicans) by using disc diffusion assay. Five 33 organic solvents ranging from non-polar to highly polar were used for extraction of active 34 metabolites. Amongst all the parts of S. xanthocarpum tested, antimicrobial activity of methanol 35 extract of fruits (14.67±0.33) against P. aeruginosa, ethanolic extract of leaves against P. 36 vulgaris (14±0.58), stem bark methanolic extract against S. aureus (13±0.58) and stem bark 37 methanolic extract against P. vulgaris (17.67±0.33) were found to be more significant. All other 38 extracts also showed promising antimicrobial activity against bacterial pathogens. Among fungal 39 pathogens, R. stolonifer and S. cerevisiae were found to be more sensitive to extracts of S. 40 xanthocarpum. Gram negative bacteria exhibited more resistance than gram positive bacteria.
41However, fungi were found to be more resistant than bacteria. All the extracts showed 42 antioxidant activity. However, methanol extract of stem bark of S. xanthocarpum with IC 50 value 43 of 0.323102 mg mL -1 showed maximum antioxidant potential. Total phenolic contents ranged 44 from 12.3541±1.73 to 23.2942±1.33 Pmol GA/ug. However, highest flavonoid content was 45 found in the stem bark extract (17.8480±1.75 ugRutin/ug) and lowest in leaves extract of S.46 xanthocarpum (2.4806±0.59 ugRutin/ug). Total metal contamination in four parts of Solanum 47 xanthocarpum (fruits, leaves, stem bark and root bark) was estimated by atomic absorption 48 spectroscopy and results showed Cadmium contamination in its stem bark and root bark, 3 49 Chromium contamination in leaves, stem bark and root bark, Copper and Magnesium 50 contamination in all parts of S. xanthocarpum and Maganese contamination in leaves critically 51 above the standard permissible limits. The proximate analysis of the Solanum xanthocarpum 52revealed that stem bark is a poor source of lipid (3.42%) and high carbohydrate (50.07%) and ash 53 (16.50%) contents. Whereas, root bark has highest wet moisture content (65%), dry moisture 54 content (20%) and lowest fiber (13%). Highest energy (285.455%), protein (8.32%), fat (9.79%) 55 and lowest amount of ash (13%) was found in fruits. This composition shows that the Solanum 56 xanthocarpum could be considered as a good source of carbohydrate, moisture and energy. 57 These results revealed that over all, methanolic extract of the S. xanthocarpum is richest in 58 phenolic, flavonoid and nutritional contents as well as most potent against bacterial and fungal 59 path...