Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-producing strain ever reported. In this paper, M(438) was identidied as Gluconacetobacter hansenii subsp. nov. on the basis of the results obtained by examining it phylogenetically, phenotypically, and physiologically-biochemically. Furthermore, the genetic diversity of strain M(438) and its initial strain was examined by amplified fragment length polymorphism. The results indicated that strain M(438) was a deletion mutant induced by HHP, and the only deleted sequence showed 99% identity with 24,917-24,723 bp in the genome sequence of Ga. hansenii ATCC23769, and the complement gene sequence was at 24,699-25,019 bp with local tag GXY_15142, which codes small multidrug resistance (SMR) protein. It can be inferred that SMR might be related to inhibiting BC production to a certain extent.
In order to improve the quality of bacterial cellulose synthesis, a Minitab-based method was proposed to analyze the factors influencing the synthesis mechanism of bacterial cellulose based on the diversity of synthetic raw materials, synthetic pathways and regulatory mechanism of bacterial cellulose. Three kinds of raw materials for bacterial cellulose production and their research status were reviewed, and the synthetic process of bacterial cellulose was optimized, which involved many related enzymes. Under these conditions, three steps of glucose hydrolysis, cellulose synthesis, final assembly and secretion were improved. The mechanism and influencing factors of bacterial cellulose synthesis were analyzed effectively, the quality of high bacterial cellulose synthesis was optimized, and the large-scale production, development and application of bacterial cellulose were prospected.
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