Gluconacetobacter hansenii subsp. nov., a High-Yield Bacterial Cellulose Producing Strain Induced by High Hydrostatic Pressure

Abstract

Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-producing strain ever reported. In this paper, M(438) was identidied as Gluconacetobacter hansenii subsp. nov. on the basis of the results obtained by examining it phylogenetically, phenotypically, and physiologically-biochemically. Furthermore, the genetic diversity of strain M(438) and its initial strain was examined by amplified fragment length polymorphism. The results indicated that strain M(438) was a deletion mutant induced by HHP, and the only deleted sequence showed 99% identity with 24,917-24,723 bp in the genome sequence of Ga. hansenii ATCC23769, and the complement gene sequence was at 24,699-25,019 bp with local tag GXY_15142, which codes small multidrug resistance (SMR) protein. It can be inferred that SMR might be related to inhibiting BC production to a certain extent.

Characterization of Bacterial Cellulose by Gluconacetobacter hansenii CGMCC 3917

“…Some studies also found that HHP treatment can bring mutagenesis to bacteria (Wang and others ; Liu and others ). HHP treatment has been successfully used to exert beneficial mutagenic effects on the BC‐producing strain and significantly increases the BC yield in our lab (Wu and others ; Ge and others ).…”

“…G. hansenii str a ins were screened in our laboratory, and then treated by HHP treatment in order to induce mutation (Ge and others ). China General Microbiological Culture Collection, Beijing, China, registered this strain as G. hansenii CGMCC3917.…”

“…China General Microbiological Culture Collection, Beijing, China, registered this strain as G. hansenii CGMCC3917. The objective of the previous studies was to optimize the production media and culture conditions for high BC yield under static conditions with the hydrolysate of waste beer yeast (WBY; Ge and others ; Lin and others ). WBY is the major by‐product from beer brewing, which is one of the largest manufacturing sectors of the food manufacturing industry in many countries.…”

Development of silver sulfadiazine loaded bacterial cellulose/sodium alginate composite films with enhanced antibacterial property

“…The method of preparing BC was well-established and described in literature (Ge et al, 2011). Briefly, in a static culture system enriched with polysaccharides, bacterial strain was incubated and was able to produce a thin layer of BC in the interface of liquid/air (Shi et al, 2012).…”

pH-responsive release behavior and anti-bacterial activity of bacterial cellulose-silver nanocomposites

“…The method of preparing BC was well-established and described in literature [26]. Briefly, in a static culture system enriched with polysaccharides (5 g/L peptone, 5 g/L yeast, 5 g/L glucose, 5 g/L mannitol and 1 g/L MgSO 4 ·7H 2 O), bacterial strain was incubated at 30 • C for 5 days and was able to produce a thin layer of BC in the interface of liquid/air [27].…”