The aim of this study was to reveal the antidiabetic and antioxidant effects of ethanolic lyophilized extract of Achillea arabica flower extract against streptozotosine (STZ)-induced in diabetic rats and to determine its phytochemical content by liquid chromatography with tandem mass spectrometry (LC-MS/MS). After toxicity test, 35 female rats were divided into five groups. Control, diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM + A. arabica (400 mg/kg) extract and DM + Glibenclamide (2 mg/kg). It was determined that while diabetic rats treated A.arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels. According to LC-MS/MS results of A. arabica flower extract, quinic acid (2439.9 μg/g), cyranoside (858.4 μg/g), chlorogenic acid (698.7 μg/g), and cosmosiin (347.8 μg/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this extract according to nuclear magnetic resonance (NMR) and Mass analyses and these compounds were named edremitine and achillosine, respectively. Thus, A.arabica flower extract has possible therapeutic effects to prevent high blood glucose level and oxidative stress caused by DM in liver and kidney via its high phenolic content.
The aim of this study was to reveal the antidiabetic and antioxidant effects of ethanolic lyophilized extract of Achillea arabica flower extract against streptozotosine (STZ)-induced in diabetic rats and to determine its phytochemical content by liquid chromatography with tandem mass spectrometry (LC-MS/MS). After toxicity test, 35 female rats were divided into 5 groups. Control, Diabetes mellitus (DM), A. arabica (400 mg/kg) extract, DM + A. arabica (400 mg/kg) extract and DM + Glibenclamide (2 mg/kg). It was determined that while diabetic rats treated A. arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels. According to LC–MS/MS results of A. arabica flower extract, quinic acid (2439.9 μg/g), cyranoside (858.4 μg/g), chlorogenic acid (698.7 μg/g) and cosmosiin (347.8 μg/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this extract according to NMR and Mass analyses and these compounds were named edremitine and achillosine, respectively. Thus, A. arabica flower extract has possible therapeutic effects to prevent high blood glucose level and oxidative stress casued by DM in liver and kidney via its high phenolic content.
Background Achillea arabica is a perennial aromatic herb widely used in folk medicine for the treatment of the stomachache and abdominal pain, wound healing, gout, cancer, diabetes and such. The aim of this study was to determine the photochemical content of the ethanolic lyophilized extract of A. arabica flower by LC-MS/MS and NMR and to reveal the antidiabetic and antioxidant effects of this extract against streptozotosine (STZ)-induced in diabetic rats.Methods and Results After toxicity test, 35 female rats were divided into 5 groups. Control, Diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM +A.arabica (400 mg/kg) extract and DM+Glibenclamide (2 mg/kg). According to LC–MS/MS results of A. arabica flower extract, quinic acid (2439.9 μg/g), cyranoside (858.4 μg/g), chlorogenic acid (698.7 μg/g) and cosmosin (347.8 μg/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this plant according to NMR analyzes and these compounds were named edremitine and achillosine, respectively. It was determined that while diabetic rats fed A. arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels.Conclusion We conclude that A. arabica flower plant extract may be a good addition to the well established pharmacological approach of DM by contributing to the reduction of overall oxidative load.
In this study, an evaluation of the protective effect of lyophilized ethanolic Achillea arabica Kotschy. extract was investigated on the islets of Langerhans in streptozotocin (STZ)-induced diabetic rats. The rats were divided randomly into 5 groups: Group I: control; Group II: rats treated with STZ (50 mg kg -1 body weight (bw)); Group III: rats treated with A. arabica (Aa) extract (400 mg kg -1 bw); Group IV: rats with DM treated with Aa extract; and Group V: rats with DM treated with glibenclamide (2 mg kg -1 bw) which is a standard drug used in the treatment of DM. STZ treatment caused histopathological changes in the islet and significantly lowered the islet diameter/area, -cell index values, and blood insulin and C-peptide levels. Administration of the extract of Aa improved the islet histology, diameter/area, and cell index values, and caused significant increases in insulin and Cpeptide levels similar to the rats treated with glibenclamide when compared to the diabetic rats. Our findings suggested that the Aa extract possessed protective potential on the pancreatic islets due to its repairing or regeneration impact on -cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.