Prolidase is a cytosolic exopeptidase that cleaves iminodipeptides with carboxy-terminal proline or hydroxyproline and plays a major role in collagen turnover. Collagen is the essential content in atherosclerotic plaque, playing a key role in the stability/ instability and progression of endothelial dysfunction in the pathogenesis of erectile dysfunction (ED). Consequently, in this study we sought to determine serum prolidase activity and markers of oxidative stress, such as lipid hydroperoxide and total free sulfhydryl, in vasculogenic ED. We evaluated 92 patients with vasculogenic ED and 50 control subjects by clinical and laboratory investigations. We measured serum prolidase activity and serum total free sulfhydryl levels spectrophotometrically. Serum lipid hydroperoxide levels were determined with ferrous ion oxidationxylenol orange method. We assessed the association of serum prolidase activity with the presence and severity of vasculogenic ED and clinical characteristics, as well as laboratory parameters. We also assessed the association of serum prolidase activity with the variables according to the vascular status of patients with vasculogenic ED. The comparison included 92 vasculogenic ED patients grouped into 3 categories according to the vascular status of patients with ED-arterial insufficiency (n 5 26), veno-occlusive dysfunction (n 5 37), and mixed-type impotence (n 5 29)-and 50 controls. Receiver-operator characteristics (ROCs) were analyzed to find a cutoff value with the best sensitivity and lowest falsepositive rate. Serum prolidase activity (53.5 6 5.5 U/L vs 45.7 6 4.9 U/L, respectively; P , .001) and serum lipid hydroperoxide levels were significantly increased in patients with vasculogenic ED compared with controls, whereas serum total free sulfhydryl levels were significantly decreased in patients with vasculogenic ED compared with controls (P , .001). The lowest and highest mean serum prolidase activities were detected in control participants and in patients with arterial insufficiency, respectively (analysis of variance P , .001). The overall findings of this study support the predictive accuracy of the serum prolidase activity in our cohort, with a statistically significant ROC value of 0.78. Findings of this study have shown that serum prolidase activity is significantly associated with the presence and severity of vasculogenic ED, and elevated serum prolidase activity might be an independent predictor of ED.

OBJECTIVES:To investigate the acute effect of phosphodiesterase type 5 (PDE5) inhibitor on erectile dysfunction by evaluating serum oxidative status and prolidase activity.METHODS:Serum samples of 36 patients with erectile dysfunction and 30 control cases were analyzed for total antioxidant status, total oxidant status, and prolidase activity, before and after the administration of tadalafil citrate.RESULTS:Before and after tadalafil citrate administration, serum total antioxidant status, total oxidant status, and prolidase were 1.1±0.0 vs. 1.6±0.0 µmol H2O2 Eq/L, 10.3±1.1 vs. 6.9±1.2 µmol H2O2 Eq/L, and 236.4±19.5 vs. 228.2±19.2 U/L, respectively (p<0.0001 for all).CONCLUSIONS:Evaluation of serum oxidative status and prolidase activity confirmed the beneficial acute effects of PDE5 inhibitor in patients with erectile dysfunction.

Sexual functionality significantly contributes to health-related quality of life and can decrease with obesity. In this study, we aimed to prospectively evaluate the changes that occur in the erection function, sexual function and testosterone level of male patients scheduled to undergo bariatric surgery, as well as the changes in the sexual function of their partner. A total of 40 patients and their partners were included. International Index of Erectile Function-5 (IIEF-5) questionnaire and the Arizona Sexual Experience Scale (ASEX) were filled before and 6 months after surgery by the male patients. The ASEX form was also completed by the partners before and 6 months after the procedure. The height and weight measurements and testosterone values were measured before and after surgery. A statistically significant difference was found between the preoperative and post-operative IIEF-5 scores of the patients (p = 0.000 < 0.01). There was also a statistically significant difference between the patients and their partners' preoperative and post-operative ASEX scores. We can conclude that the partners of men with preoperative erection complaints also experience sexual dysfunction, and with the post-operative decrease in or disappearance of erection complaints, the sexual function of both male patients and their partners improves.

Despite of the documented strong inverse relationships between Diabetes Mellitus (DM) and male/female fertility, and also between IR and female infertility via ovarian functions as in polycystic ovary syndrome, to our knowledge, there is no report about any influence of IR on male fertility. DM and metabolic syndrome (MetS) have negative influence on fertility. Thus, IR may be accused of causing detrimental effect on male infertility due to hyperinsulinemic state and being one of the components for MetS. Interestingly, due to our preliminary results, we do not found any inverse correlation between IR and male reproductive functions.

PurposeThe aim of this study, to investigate whether there is any association between enuresis in childhood and nocturnal polyuria syndrome (NPS) in adult life.MethodsThe study consisted of thirty five patients with nocturnal polyuria, and thirty five healthy people without nocturnal polyuria in adult life, were asked to assess their enuresis in childhood.ResultsThere was a history of enuresis in childhood in 18 (51.42%) of 35 of men with nocturnal polyuria and in 4 (11.42%) of 35 without nocturnal polyuria. Enuresis in childhood was significantly more common in men with nocturnal polyuria than without nocturnal polyuria. The difference was significant (P<0.0001). The prevalence of enuresis in the nocturnal polyuria (51.42%) was more than two-fold higher than reported prevalence in general populations.ConclusionsThe results of this study suggest that the history of enuresis in childhood seems to increase the risk of having NPS in adult life. This relationship should be taken into account in the evaluation of men with complaints from NPS in adult life and the possible common pathophysiology should be considered in the treatment planning.

We studied the role of the resting period (1, 2, 4, 8, 16 min; n = 6-7), external Ca2+ (0.2, 0.4, 0.6 g/l; n + 5-6), stimulation frequency (1, 2, 3 Hz; n = 6), 4-aminopyridine (4-AP, 2 mM; n = 5); theophylline (1 mM; n = 6), ouabain (5 microM; n = 6), and verapamil (1 microM; n = 6) on post-rest adaptation in the isolated left atria of rats driven electrically by a 2x threshold intensity for 2 ms. Resting periods resulted in three-phasic adaptive changes in contractility during the post-rest stimulation before normalization: P1, hypercontractile phase, an initial twitch potentiation; P2, post-rest hypocontractile decay reached after 8 to 12 single twitches; and P3, a late reactive hypercontractile phase marked less than that of P1 and gradually declining to the pre-resting level. P1 and P2 were augmented along with increasing the resting period from 1 min to 16 min, whereas t1 (time between P1 and P2) shortened and P2 and t2 (time between P2 and P3) were not affected. P1 and P3 to become more apparent after shifting the stimulation frequency from 1 Hz to 3 Hz, accompanied by a shortening of t1 and t2 (p < 0.05) and an insignificant reversal of P2. An increase in Ca2+ concentration by 2- or 3-fold at 2 Hz reduced P1 was and antagonized P2, while leaving other parameters almost unaffected. The reduction of P1 by Ca2+ became more prominent at 3 Hz. Exposure to 4-AP depressed P1 and P3 at 1 Hz, which was reversed by increasing the stimulation frequency--P3 tended to diminish, whereas t1 and t2 were shortened. Theophylline reduced P1 antagonized P2, and shortened t1 and t2 significantly, and a combination of theophylline and 4-AP augmented the effects. Ouabain increased P1 and P2 in a frequency-dependent manner; prolonged t2 at 1 Hz, but shortened t2 at higher frequencies. Verapamil inhibition of Ca2+ channels augmented P1 and t1 and reduced P2 and P3, and the effects on all three parameters were augmented by combined 4-AP/verapamil. We concluded that the post-rest adaptive changes in contractility are a consequence of phasic changes in sarcoplasmic Ca2+ concentration and that such changes reflect an imbalance between the release from and uptake into the sarcoplasmic reticulum of Ca2+ and transsarcolemmal Ca2+ loss.

Penile prosthesis implantation (PPI) is the final stage treatment in erectile dysfunction (ED). In this study, we planned to investigate the effect of PPI application on sexual functionality in the patients and their partners. After taking permission and consent for the study, from 20 male patients who were applied penile prosthesis due to ED between March 2013 and June 2018 and their partners, the couples were included in the study. Patients and partners filled in Arizona Sexual Experiences Scale (ASEX) form before PPI. After starting to use prosthesis, 20 patients and 19 partners were asked to fill in modified EDITS and ASEX form in the follow‐ups in the sixth month. Average age was 54.35 years for the patients and 43.84 for the partners. Although post‐PPI sexual satisfaction ratio was detected higher in female partners compared with the male patients, this difference was not statistically significant (p = .71). A significant recovery was also observed in total scale score, physiological stimulation, orgasm capacity and satisfaction scores in both groups after PPI. Penile prosthesis implantation is an operation providing high satisfaction for both the partner and the patient and is still one of the best options of ED.

Objective: Epidemiological and experimental studies indicate that kidney disease is associated with increased oxidative stress. Our aim was to determine whether paraoxonase 1 (PON1) activity is altered in patients with moderately decreased glomerular filtration rates (GFRs) compared to healthy controls. Material and methods: Forty-eight patients showing relatively low GFRs upon renal scintigraphy with 99m Tc-DTPA were compared to 40 age-matched healthy subjects. Serum PON1 activity was measured spectrophotometrically. Lipid hydroperoxide levels were measured via iodometric assay. Results: The mean ages of the patient and control groups were 32.09 ± 6.10 (range 23-50) and 31.30 ± 5.30 (range 20-46) years, respectively. Serum PON1 (p = 0.949) and high-density lipoprotein (p = 0.473) levels did not differ between groups. Significant differences were detected between groups in terms of mean triglyceride (p = 0.009), very-low-density lipoprotein (p = 0.010), lipid hydroperoxide (p = 0.026), urea (p = 0.012), and creatinine (p = 0.001) levels, whereas total cholesterol (p = 0.520) and low-density lipoprotein (p = 0.161) were similar between groups. Mean GFR was significantly lower in the low GFR group compared to the control (p = 0.000). Conclusion: Our results indicate that PON1 activity and high-density lipoprotein levels may not be determining factors in premature vascular aging in patients with moderately decreased GFRs. Instead, some other undetermined factor(s) may be involved in modulating enzymatic activity.