Serum paraoxonase activity in patients with low glomerular filtration rates

Abstract

Objective: Epidemiological and experimental studies indicate that kidney disease is associated with increased oxidative stress. Our aim was to determine whether paraoxonase 1 (PON1) activity is altered in patients with moderately decreased glomerular filtration rates (GFRs) compared to healthy controls. Material and methods: Forty-eight patients showing relatively low GFRs upon renal scintigraphy with 99m Tc-DTPA were compared to 40 age-matched healthy subjects. Serum PON1 activity was measured spectrophotometrically. Lipid hydroperoxide levels were measured via iodometric assay. Results: The mean ages of the patient and control groups were 32.09 ± 6.10 (range 23-50) and 31.30 ± 5.30 (range 20-46) years, respectively. Serum PON1 (p = 0.949) and high-density lipoprotein (p = 0.473) levels did not differ between groups. Significant differences were detected between groups in terms of mean triglyceride (p = 0.009), very-low-density lipoprotein (p = 0.010), lipid hydroperoxide (p = 0.026), urea (p = 0.012), and creatinine (p = 0.001) levels, whereas total cholesterol (p = 0.520) and low-density lipoprotein (p = 0.161) were similar between groups. Mean GFR was significantly lower in the low GFR group compared to the control (p = 0.000). Conclusion: Our results indicate that PON1 activity and high-density lipoprotein levels may not be determining factors in premature vascular aging in patients with moderately decreased GFRs. Instead, some other undetermined factor(s) may be involved in modulating enzymatic activity.

Validation of spectrophotometric assays for serum paraoxonase type-1 measurement in dogs

“…1 This enzyme has received increasing attention in human medicine, largely because alterations in circulating PON1 are associated with various diseases involving oxidative stress, such as renal or liver disease or neoplasia. [2][3][4] In addition, PON1 has anti-inflammatory properties. 5 The activity of PON1 is reportedly low in humans with type 2 diabetes mellitus 6 and obesity, 7,8 and low activity may contribute to the development of cardiovascular disease.…”