Harmful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species. Maximum growth rates were 0.59 ± 0.08 d−1 for O. cf. ovata, 0.35 ± 0.01 d−1 for C. monotis and 0.33 ± 0.04 d−1 for P. lima. Toxin analyses revealed the presence of ovatoxin-a and ovatoxin-b in O. cf. ovata cells. Okadaic acid and dinophysistoxin-1 were detected in P. lima cultures. For C. monotis, a chromatographic peak at 5.6 min with a mass m/z = 1061.768 was observed, but did not correspond to a mono-sulfated analogue of the yessotoxin. A comparison of the toxicity and growth characteristics of these dinoflagellates, distributed worldwide, is proposed.

Long-term fire history reconstructions provide fruitful information in the context of global change. Global remotely-sensed burned areas offer a uniform estimate of fire regimes worldwide, but hardly capture small fire events and cover only the last 20 years. Burned areas from national statistics often lack credibility due to discrepancies in fire report protocols between countries, partial data records and uncertain burned area estimates from field observations. However, they constitute a unique and valuable alternative long-term key source of information. We provide here a detailed critical analysis of the fire database in Tunisia, on the southern boundary of the Mediterranean basin and with a contrasted socio-economic environment compared to the more studied European side. We analyzed the fire record database with a quality checking protocol, combined with remote sensing burned area characterization from Landsat images. The high uncertainties in fire numbers could not lead to any conclusion for an accurate trend estimate. The corrected burned area lead to an average yearly burned area of 1799 ha year −1 compared to previous estimates of 1017 ha year −1 , leading to a fraction of burnable land affected by fires of 0.19%, on the lowest range of observations in the Mediterranean basin. From this corrected database, we revised the usually assumed burned area decrease in this region, with no significant trend detected over the 1985-2010 period. We conclude on the need for thorough assessment of data quality in fire history reconstruction from national statistics to prevent misleading conclusions, and for an increased credibility, in order to be further used in fire models benchmarking or fire weather analysis. Our results can contribute to the under-represented fire regime analysis on the southern boundary of the Mediterranean basin.

Macrophytes are known to release allelochemicals that have the ability to inhibit the proliferation of their competitors. Here, we investigated the effects of the fresh leaves of two magnoliophytes (Zostera noltei and Cymodocea nodosa) and thalli of the macroalgae Ulva rigida on three HAB-forming benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima, and Coolia monotis). The effects of C. nodosa and U. rigida were also tested against the neurotoxic planktonic dinoflagellate Alexandrium pacificum Litaker sp. nov (former Alexandrium catenella). Co-culture experiments were conducted under controlled laboratory conditions and potential allelopathic effects of the macrophytes on the growth, photosynthesis and toxin production of the targeted dinoflagellates were evaluated. Results showed that U. rigida had the strongest algicidal effect and that the planktonic A. pacificum was the most vulnerable species. Benthic dinoflagellates seemed more tolerant to potential allelochemicals produced by macrophytes. Depending on the dinoflagellate/macrophyte pairs and the weight of leaves/thalli tested, the studied physiological processes were moderately to heavily altered. Our results suggest that the allelopathic activity of the macrophytes could influence the development of HAB species.

A study of pesticides in the Bizerte lagoon watershed on the Mediterranean coast of Tunisia 16 showed that herbicides and fungicides are the most commonly used compounds. A survey 17 was made of selected farmers. Pesticide contamination was monitored in the water column 18 and sediments at four selected sampling sites (lagoon (A) and in three oueds: Chegui (B), 19 Garaa (C) and Tinja (D)). Polar organic chemical integrative samplers (POCIS) were used to 20 assess pesticide contamination. Thirty-two pesticides were investigated; the total

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