In our previous work we found the presence of ring-shaped nuclei in circulating neutrophils of healthy individuals whose nuclei were stained only by means of the Feulgen reaction [1]. In our present work, we performed another study on the blood of 52 healthy human donors and stained their blood films with hematoxylin. In all cases, ring-shaped nuclei were revealed. In this series, the number of this type of nuclei was high: 15.61% +/- SD 7.18%. The ring nuclei of healthy humans had thin segments, such as we have previously found by means of Feulgen method.
branch D. limbata biosynthesizes polygodial (1), which constitutes its chemical defense and is stored in the mantle. Thus the ability of a nudibranch to elaborate its chemical defense has been demonstrated. Dendrodoris limbata also synthesizes the mixture of sesquiterpenoid esters (2) found in the digestive glands. We believe that these esters should be regarded as products of further metabolism of polygodial as a result of a detoxication process. Polygodial is a reactive molecule that readily interacts with NH2 groups (16) and therefore could be toxic for D. limbata itself if stored for a long time. When the animal is molested, the compound is secreted through the skin. The esters (2), which are found into the hepatopancreas only, could represent the normal excretory metabolites of polygodial.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.