Ring‐shaped nuclei in human neutrophilic leukocytes of healthy individuals: Evidence of their occurrence and characteristics

Cabral, H. R. A.; Robert, Gabriela B.

doi:10.1002/ajh.2830300414

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…The nuclei of normal human neutrophils may share an annular shape with several species, like rats and mice, as observed by Cabral and Robert [15] . Stavem et al [16] re- ported on ring-shaped nuclei of human granulocytes in acute erythroleukemia.…”

Section: Nuclei Of Neutrophils Achieve Segmentationmentioning

confidence: 68%

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Chaudhuri¹,

Walther²

2012

Acta Haematol

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Segmentation, condensation and bilateral symmetry of the nuclei of polymorphonuclear leukocytes seem related to their function. Segmentation of the nuclei into two or more lobes and their condensation facilitate their passage (diapedesis) through the endothelial layer of blood vessels to the extravasal space and subsequent locomotion through the interstitial compartment of different tissues. Bilateral symmetry of these nuclei along with their association to the cytoskeletal fibers contribute to their efficiency in locomotion by alignment of the axis of nuclear symmetry to the axis of cellular polarity, which orients towards the direction of locomotion in response to cytokines and other stimuli. Observations of the cytogenetic facets of intranuclear order support these assumptions.

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Section: Nuclei Of Neutrophils Achieve Segmentationmentioning

confidence: 68%

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Chaudhuri¹,

Walther²

2012

Acta Haematol

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“…The nuclei of normal human neutrophils with two or more lobes may also share an annular shape 6 with several other species, like rats and mice. Ring-shaped nuclei of human granulocytes in acute erythroleukemia were seen earlier.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte Nucleus Reveals a Linear Order of Chromosomes Separated in Two Parental Genomes That Favors the Process of Gene Activation

Chaudhuri

Karamanov

Scott³

et al. 2018

J Histochem Cytochem.

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Analysis of trisomy 8 cells and the chromosome-specific fluorescence in situ hybridization (FISH) signals on the ring-shaped nucleus of a neutrophil reveal that homologue chromosomes orient in diametrical opposition to each other. This positioning results in a separation of the two haploid sets of parental chromosomes organized as two exclusive groups. These two groups impart the nucleus a symmetry that fortifies immune protection by accelerating chemotaxis. The ring form of the nucleus is a legacy of the orientation of chromosomes as a rosette during metaphase and telophase stages. A dual control maintains this spatial order: (1) chromosomes are tethered to the centriole all through the cell cycle, and (2) during their circular orientation in telophase the chromosomes bind to each other with lamins, which reorganize the nuclear membrane of the daughter nuclei, generating an additional anchorage. Here, chromosomes serve as temporary packets to assure proper distribution of the nuclear DNA during mitosis. The remainder time of the cell cycle the chromosomes are chained together across the telomeres, allowing a continuous sequence of genes of the two genomes, maternal and paternal, thus facilitating easy reading of the gene sequence. Exceptions to these orders are either physiological and temporary, or pathological and disease causing.

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“…The percentage of band cells increase during infection . In addition, ring‐shaped neutrophils have been identified in healthy donors . Eosinophils are the same size as neutrophils or slightly larger.…”

Section: Bacteria Biofilm and Chronic Bacterial Infectionsmentioning

confidence: 99%

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

Alhede

Stavnsbjerg

Bjarnsholt

2018

APMIS

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The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

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Ring‐shaped nuclei in human neutrophilic leukocytes of healthy individuals: Evidence of their occurrence and characteristics

Cited by 6 publications

References 4 publications

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Nuclear Segmentation, Condensation and Bilateral Symmetry in Polymorphonuclear Leukocytes Reflect Genomic Order and Favor Immunologic Function

Leukocyte Nucleus Reveals a Linear Order of Chromosomes Separated in Two Parental Genomes That Favors the Process of Gene Activation

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

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