The Fc-C'-receptor-positive cells were investigated in tumor cell suspensions from 196 malignant tumors. The number of EA (Fc) and EAC' (C') rosettes was determined; the rosetteforming cells were identified cytologically, the cell suspensions were exposed to carbon particles, and they were grown in tissue culture. The results suggest that Fc or C' receptors might be present in malignant tumor cells as well as in host defense (inflammatory) cells invading the tumor.Cancer 48 pensions to carbon particles in order to functionally identify the macrophages. The results suggest that there are Fc or C' receptors in the surface membrane of malignant tumor cells other than those found in malignant lymphoma or leukemia.
Materials a n d MethodsCell suspensions from 196 malignant tumors were prepared from fresh intraoperatively obtained tumor tissue. The cells were collected in TNG (tris-NaC1-glucose, p H 7.3; Merck, Darmstadt) which was supplemented by I mg DNAse (Boehringer, Mannheim). After centrifugation, the pellet was resuspended in 10 ml red blood cell remover, centrifuged, and resuspended in TNG.The red blood cell remover was composed of three solutions designated A, B, and C. Solution A consisted of 35.0 g of NH4CI; 1.85 g of KCl; 1.550 g of NaHPO, x 2 H,O; 0.119 g of KH,PO,; 5.0 g of glucose; 0.05 g of phenolrot -+ ad 1000 ml of aqua tridest. Solution B consisted of 0.42 g of MgC& x 6 H 2 0 ; 0.14 g of MgSO, x 7 H,O .+ ad 1000 ml of aqua tridest. Solution C consisted of 2.25 g of NaHCO, + ad 100 ml of aqua tridest. The red blood cell remover was made up of 2 ml of solution A, 0.5 ml of solution B, and 0.5 ml of solution C + ad 10 ml aqua tridest. TNG contains 15% fetal calf serum (FCS) (Flow) and 0.2 mg of DNAse. The number of cells was adjusted to 1 x 106/ml 15% TNG-FCS-DNAse.Preparation of Assay Cells for Fc Receptor (EA)J First, 250 pl of sheep red blood cells (SRBC) were suspended in 1 ml of PBS (pH, 7.2) and 1 ml of 0.1%
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