Hormonabh�ngige Enzymverteilung in Geweben

Localization of d-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase in rat kidney

“…The enzymic results could therefore be explained by assuming that the inositol 1: 2-cyclic phosphate 2-phosphohydrolase is confined to the pars convoluta whereas the alkaline phosphatase, although also present in the convoluted portion, extends much further along the tubule into the pars recta and maybe even into the loop of Henle. A number of histochemical studies have indicated that in the rat alkaline phosphatase extends throughout the proximal tubule (Longley & Fisher, 1954;Baumann et al, 1964;, which is in contrast with that of the mouse nephron (Hardonk & Koudstaal, 1968). Schmidt & Dubach (1968) by using a microdissection technique have measured the distribution of alkaline phosphatase along the proximal convolution of the rat kidney and have found that even with this enzyme the distribution and total activity can vary between a short (superficial) and a long (juxtamedullary) nephron.…”

A comparison of d-inositol 1:2-cyclic phosphate 2-phosphohydrolase with other phosphodiesterases of kidney

“…In the primary cell fractionation of rat kidney cortex, activities for breakdown of all the phosphodiester substrates apart from the cyclic adenosine monophosphates had a distribution similar to that for glycerol 2-phosphate (alkaline phosphatase) with maximum specific activity occurring in the 'light mitochondrial' fraction. Isolation of brush-border fragments (Wilfong & Neville, 1970) and histochemistry Baumann et al, 1964) have indicated that in the rat kidney alkaline phosphatase is located in the brush borders of the tubules. Breakdown of 3':5'-cyclic AMP occurred predominantly in the supernatant fraction from the kidney, confirming the observations of Dousa & Rychlik (1970) and Jard & Bernard (1970).…”

“…4) shows a close resemblance to that of alkaline phosphatase, which in the adult mouse is believed from histochemical observation to be largely located in the brush borders of the pars convoluta (Hardonk & Koudstaal, 1968). In the rat kidney, however, alkaline phosphatase, which occurs throughout the brush borders of the proximal tubule (Longley & Fisher, 1954;Baumann et al, 1964;, extends right through the cortex and much farther into the kidney than inositol 1:2-cyclic phosphate 2-phosphohydrolase (Fig. 3).…”

Histochemistry and Cell Biology—a glance into the past and a look ahead

“…In contrast, ovarectomy resulted in a strong reduction of enzyme activity without an additional effect of subsequent testosterone treatment (Baumann et al. 1964 ). Analogous studies and experiments in mice revealed sex-related differences for alkaline phosphatase and glucose-6-phosphatase activity (von Deimling et al.…”