The objective of the current study was the development of a simple, rapid, and accurate isocratic reverse-phase ultra-performance liquid chromatographic (RP-UPLC) method for the routine control analysis of imipramine hydrochloride (IMH) in bulk drug and in pharmaceutical formulations. This work was carried out in order to reduce analysis time and maintaining good efficiency which in turn is focused on high-speed chromatographic separations. The method was developed using Waters Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with mobile phase consisting of a mixture of acetonitrile and ammonium acetate buffer of pH-5 (80 : 20, v/v/v). UV detection was performed at 220 nm for eluted compound. An excellent linearity was observed in the concentration range 0.2–3 µg/mL IMH with a regression coefficient () value of 0.9999. The method developed was validated and forced degradation was performed as per ICH guidelines. The limit of detection () was 0.2532 ng/mL and the limit of quantitation () was found to be 0.7672 ng/mL. The drug IMH was subjected to hydrolytic, acidic, basic, thermal, photolytic, and oxidative stress conditions according to ICH regulations. IMH was found to be stable in basic, thermal, and photolytic conditions and degrades in acidic, hydrolytic, and oxidative stress conditions.
Ascorbic acid is one of the major water soluble vitamin and many health benefits have been attributed to ascorbic acid such as antioxidant, anti-atherogenic,immunomodulator anti-carcinogenic etc. It plays a vital role in the biosynthesis of collagen, carnitine and neurotransmitters. As humans can not produce ascorbic acid due to the lack of an enzyme gulonolactone oxidase, it has to be supplemented mainly through fruits, vegetables and tablets. In this present work, we have reported a simple, cost effective, reliable titrimetric method for the estimation of ascorbic acid present in commercially available Vit-C tablets and also the determination of molecular weight of ascorbic acid. The method involves the oxidative dehydrogenation of ascorbic acid by potassium iodate followed by the determination of unreacted potassium iodate by iodometry.
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