The response of adrenal glands of Single Comb White Leghorn laying hens housed under different population densities was studied. The birds were reared in floor pens until they were 19 weeks of age, after which they were housed in cages. Cage size was 30.5 X 50.8 cm. Three, four, or five birds were housed per cage. Corticosterone concentrations were measured in all birds 48 and 96 hr following housing in cages and weekly thereafter for 6 weeks. Corticosterone concentrations were consistently higher in the serum of birds housed five per cage than in birds housed three or four per cage. It was concluded that in order to minimize physiological stress in cage layers, more than 387 cm2 per bird should be considered.
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