a b s t r a c tA study was carried out to examine if the amine ( NH 2 ) group located on the side chains of lysine (K), glutamine (Q), or asparagine (N) residue has any effect on the macrocyclization of b ions even though the N-terminals of the peptides were acetylated. The work utilized the model peptides Ac-KYAGFLVG, Ac-QYAGFLV-NH 2 , and Ac-NYAGFLV-NH 2 . The CID mass spectra of b 7 ions originated from these three peptides exhibited that the macrocyclization still occurred for the lysine containing peptide in spite of the N-terminal of the peptide was acetylated, but was failed to be observed for glutamine and asparagine containing peptides. These current results reveal that the lysine side chain -amine group has been involved in the macrocyclization of the peptide b ions for the N-terminal acetylated peptides and consequently, non-direct sequence b ions were observed in the CID mass spectra. However, due to the amide group on the side chains of the glutamine and asparagine residues, the nucleophilicity of their groups greatly reduced; therefore the scrambling b ions were not detected in their b 7 ion CID mass spectra. In addition, the effect of the lysine position was also studied for series of six isomeric octapeptides such as, Ac-KYAGFLVG, Ac-YKAGFLVG, Ac-YAKGFLVG, Ac-YAGKFLVG, Ac-YAGFKLVG and Ac-YAGFLKVG in order to examine the relationship between the intensities of non-direct sequence b ions and the lysine position in the octapeptide series. The results clearly demonstrated that the most abundant non-direct sequence b ions were observed for the first position of lysine residue in the N-terminal acetylated octapeptide, however, when the lysine residue gets closer to the C-terminal position the relative intensities of the scrambled b ions were greatly decreased.

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A novel ligand, N, N 2-(4-methyl-1,2-phenylene)bis(2-(biphenyl-4-yl)-N 1-hydroxy-2-oxoacetimidamide) (H2L) with its Cu(II) and Mn(II) complexes were synthesized in this study. All compounds synthesized were also characterized by 1 Hand 13 C-NMR, the Fourier transform infrared, elemental analysis, inductively coupled plasma optical emission spectrometry, molar conductivity, magnetic susceptibility measurements and thermogravimetric analysis. Vascular endothelial growth factor-2 (VEGFR-2) and cyclooxygenase-2 (COX-2) inhibition is often used as a parameter for being a potent anticancer agent in docking studies. For this purpose, synthesized and characterized ligand was investigated by molecular docking study to test its inhibitory effect against angiogenic factors VEGFR-2 and COX-2.

a b s t r a c tA systematic study was carried out to examine the effects of the side chain, peptide size, residue position, and neighboring amino acid on the macrocyclization of b ions. The work utilized isomeric model peptides YAGFLV-NH 2 , AGFLVY-NH 2 , GFLVYA-NH 2 , FLVYAG-NH 2 , LVYAGF-NH 2 , VYAGFL-NH 2 , which all have the same amino acid sequence in cyclic form. The b 6 ions derived from all these isomeric peptides form the same macrocyclic structure due to the generation of the same amino acid sequence order upon cyclization. Hence, the MS/MS spectra and breakdown graphs of b 6 ions derived from these peptides are similar to each other. However, the relative intensities of the non-direct sequence ions in both the MS/MS spectra and breakdown graphs of the b 6 ions derived from FAYVGL-NH 2 , GVYALF-NH 2 and VFYLAG-NH 2 show a different distribution from each other and the first series, even though they are all isomeric peptides. This could be due to the different amino acid sequence order in the cyclic forms of these peptides. It is clearly shown that the neighboring amino acid influences the selective opening of the macrocyclic form.Additionally, XYAGFLV-NH 2 and YAGXFLV-NH 2 (where X = C, D, E, H, K, M, N, P, Q, S, T, and W are amino acid residues) were also studied in order to examine the influence of the peptide size, amino acid side chain, and position on the ring formation and cleavage of macrocyclic b 5 , b 6 and b 7 ions. The results have clearly shown that b 6 and b 7 ions have a higher tendency of macrocyclization compared to b 5 ions with the exception of QYAGFLV-NH 2 . Additionally, it was observed that selective ring opening is also dependent on the size of the b ions and the position of the amino acid residue. From our study of the macrocyclic b 6 ions of our model peptides, the Q, W, K, and M residues were found to be more favorable eliminations when compared to C, D, E, H, N, P, S, and T. Based on the results, no preferential cleavage order can be specified depending on the nature of amino acid side chain.

2019 Koronavirüs (COVID-19) Pandemisi insanların sağlık bilgilerini edinmelerini, uygulamalarını ve davranışlarını hızlı bir şekilde edindikleri bilgilere uyarlamalarını gerektirmiştir. Bireylerin sağlıkla ilgili konularda bilinçlendirilmesi, sağlık okuryazarı bireyler yetiştirilmesi ile mümkün olacaktır. Yapılan çalışmalar sağlık okuryazarlığı temellerinin atıldığı dersin, içeriği ve kazanımları göz önünde bulundurulduğunda fen bilimleri dersi olduğunu göstermektedir. Fen bilimleri öğretmenlerinin kendi sağlıklarını etkileyen faktörlere karşı bilinçli ve iyi birer sağlık okuryazarı olmalarının öğrencilerinin de sağlık okuryazarı olma durumlarını etkileyeceği düşünülmektedir. Fen bilimleri öğretmenlerinin de sağlık okuryazarlığı becerilerine sahip olabilmesi için lisans düzeyinde donanımlarını arttırmaları gerekmektedir. Bu kapsamda fen bilimleri öğretmen adaylarının sağlık okuryazarlığı durumlarının, sağlık okuryazarlığına ilişkin görüşlerinin ve farkındalıklarının belirlenmesi amacıyla mevcut çalışma gerçekleştirilmiştir. Çalışmada nitel araştırma yöntemlerinden iç-içe geçmiş tek durum deseni kullanılmıştır. Bu doğrultuda 2020 yılı bahar döneminde Burdur Mehmet Akif Ersoy Üniversitesi, Eğitim Fakültesi, Fen Bilgisi Eğitimi Anabilim Dalı’nda öğrenim gören 3. ve 4. sınıf öğrencilerinden toplam 12 gönüllü öğrenci ile çalışma yürütülmüştür. Veri toplama aracı olarak yarı yapılandırılmış görüşme formu kullanılmıştır. Çalışmadan elde edilen veriler doğrultusunda fen bilimleri öğretmen adaylarının beslenme ve iyi sağlık halinin ilişkisinin farkında oldukları, ilaç kullanımına ilişkin bilinçli davrandıkları, sağlık hizmetlerinden yararlanma bilinçlerinin yüksek olduğu, ilk yardımın insan hayatı için öneminin farkında oldukları sonucuna ulaşılmıştır.

A novel aminoketooxime ligand and its mononuclear Cu(II) and Mn(II) complexes were synthesized. The structures of the synthesized compounds were illuminated by elemental analysis, inductively coupled plasma optical emission spectrometry, the Fourier transform infrared, 1 H-and 13 C-NMR, UV-Vis, magnetic susceptibility and conductivity measurements. According to the characterization studies ligand to metal ratio was found to be 2:1 with strong binding affinity of the ligand to the metal ions. In addition, complexes have been tested for their catecholase and phenoxazinone synthase-like activities. Kinetic studies were also carried out yielding Vmax, kcat and KM values of both complexes for catecholase and phenoxazinone synthase-like activities. Both complexes efficiently catalyzed the reactions of the enzymes they mimicked.

Two novel Schiff base ligands, 2, 2 I -((pentane-2,4-diylidene)bis(azanylylidene))bis(4-chlorophenol) and 2, 2 I -((pentane-2,4-diylidene)bis(azanylylidene))bis(4-methylphenol) were prepared by condensation reaction of acetylacetone with 2-amino-4-chlorophenol and 2-amino-4-methylphenol, respectively. Mononuclear Cu(II) complex of each ligand was also synthesized. These compounds were characterized by elemental analyses, inductively coupled plasma optical emission spectrometry, the Fourier transform infrared spectra, UV-Vis, molar conductivity, and magnetic susceptibility measurements. The ligands have been further characterized by 1 H-and 13 C-NMR. The results suggest that the metal complexes of the ligands have a metal:ligand ratio of 1:1 and the Cu(II) ions are coordinated by the phenolic oxygen atoms and imine nitrogen atoms of the ligands. Moreover, the quantum chemical calculations of the compounds have been performed using the density functional theory. It has been found that the calculated spectroscopic values are in a good agreement with experimental data.