The angular resolution of many commercial imaging systems is limited, not by diffraction or optical aberrations, but by pixilation effects. Multiaperture imaging has previously demonstrated the potential for super-resolution (SR) imaging using a lenslet array and single detector array. We describe the practical demonstration of SR imaging using an array of 25 independent commercial-off-the-shelf cameras. This technique demonstrates the potential for increasing the angular resolution toward the diffraction limit, but without the limit on angular resolution imposed by the use of a single detector array.
Traditional approaches to imaging require that an increase in depth of field is associated with a reduction in numerical aperture, and hence with a reduction in resolution and optical throughput. In their seminal work, Dowski and Cathey reported how the asymmetric point-spread function generated by a cubic-phase aberration encodes the detected image such that digital recovery can yield images with an extended depth of field without sacrificing resolution [Appl. Opt. 34, 1859(1995]. Unfortunately recovered images are generally visibly degraded by artifacts arising from subtle variations in point-spread functions with defocus. We report a technique that involves determination of the spatially variant translation of image components that accompanies defocus to enable determination of spatially variant defocus. This in turn enables recovery of artifact-free, extended depth-of-field images together with a two-dimensional defocus and range map of the imaged scene. We demonstrate the technique for high-quality macroscopic and microscopic imaging of scenes presenting an extended defocus of up to two waves, and for generation of defocus maps with an uncertainty of 0.036 waves.
A new single-aperture 3D particle-localization and tracking technique is presented that demonstrates an increase in depth range by more than an order of magnitude without compromising optical resolution and throughput. We exploit the extended depth range and depth-dependent translation of an Airy-beam PSF for 3D localization over an extended volume in a single snapshot. The technique is applicable to all bright-field and fluorescence modalities for particle localization and tracking, ranging from super-resolution microscopy through to the tracking of fluorescent beads and endogenous particles within cells. We demonstrate and validate its application to real-time 3D velocity imaging of fluid flow in capillaries using fluorescent tracer beads. An axial localization precision of 50 nm was obtained over a depth range of 120μm using a 0.4NA, 20× microscope objective. We believe this to be the highest ratio of axial range-to-precision reported to date.
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