Chemical investigation of the ethanol extract of propolis allowed the isolation of 2'-hydroxy-4',7-dimethoxyisoflavan (1) 2',7-dihydroxy-4'-methoxyisoflavan (2) 2',4'-dihydroxy-7-methoxyisoflavan (3), 4',7-dihydroxy-2'-methoxyisoflavan (4) 2',4',4-trihydroxychalcone (5) and lup-20(29)-en-3-ol (6). The structures of compounds were identified based on their spectral data and by comparison with the literature. It was performed test to evaluate antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method of the ethanol extract and its fractions obtaining significant results similar to Vitamin C. It was also evaluated anticholinesterase activity, observing promising results with the extract and fractions. Being the red propolis of these activities, it can be used in the treatment of degenerative diseases such as Alzheimer. There are already records of propolis as an antioxidant, however, the activity of anticholinesterase is being cited for the first time.
Objective:
To evaluate the effect of red propolis and
L
-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells.
Methods:
The study consisted of two experiments with four groups each (total: 57 hamsters). In the experiment 1, the animals were inoculated with Walker tumor cells, followed by administration of test substances (red propolis 200mg/5mL/kg or
L
-lysine 150mg/kg) or control substances (gum arabic 5mL/kg or water 5mL/kg) for 10 days. The animals in the experiment 2 received red propolis,
L
-lysine, gum arabic or water at the same doses, for 33 days prior to inoculation of Walker tumor cells, followed by 10 days of treatment with the same substances. Based on single-plane images, angiogenesis was quantified (mean vascular area), in percentage, and tumor area (mm
2
) and perimeter (mm).
Results:
In the experiment 1, compared to animals receiving water, the mean vascular area expressed in percentage was significantly smaller in animal treated with propolis (p<0.05) and
L
-lysine (p<0.001).
Conclusion:
Both red propolis and
L
-lysine inhibited tumor angiogenesis in the new hamster cheek pouch model when administered after tumor inoculation.
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