Affinity purification of annexin V from human placenta on column with appropriate monospecific antibodies is developed. The procedure permits purification of the protein to a highly purified state by a two stage procedure. The yield of the protein is about 5 mg per 100 g of wet tissue. Because of high homologies between various annexins, it was supposed that this procedure can be also applied for purification of other annexins from other tissues.
Anisotropic silver nanoparticles sensitized by various lectins were applied for detection of yeast (Saccharomyces cerevisiae) which was used as a model of fungal cells.
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