A Photometric Approach for Detection of Interaction of Cells with Their Ligands Based on Lectin Conjugated Gold Nanoparticles

Mikaelyan, Mariam V.; Poghosyan, Gayane G.; Gasparyan, Vardan K.

doi:10.1080/00032710802052577

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Therefore, it is reasonable to expect that any interactions of these nanoparticles with various ligands or cells will bring changes in their optical properties. This property was applied successfully for the detection of protein-protein, protein cell, and protein-bacteria interaction [30][31][32]. Moreover such nanoparticles have high affinity to SH groups and therefore they can be functionalized easily for consequent covalent immobilization of various molecules.…”

Section: Resultsmentioning

confidence: 99%

Selective interaction of some glyconanoparticles with stomach and intestine cancer cells

Gasparyan

Khachatryan

Badalyan

2023

Preprint

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Spherical nanoparticles of silver were sensitized by glucose, lactose, dextran, and chitosan. Interactions of these nanoparticles with normal and cancerous tissues of the stomach and the intestine were studied. It was shown that nanoparticles sensitized with lactose interact selectively with cancer cells of the intestine, whereas glucose or dextran-sensitized nanoparticles interact selectively with stomach cancer cells. However, it should be noted that all of these nanoparticles interact to some extent with normal cells, also. Such nanoparticles loaded with anti-cancer drugs may be applied for selective action to these cancer cells

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Section: Resultsmentioning

confidence: 99%

Selective interaction of some glyconanoparticles with stomach and intestine cancer cells

Gasparyan

Khachatryan

Badalyan

2023

Preprint

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“…This approach permitted to detect the presence of 3000 lymphocytes/ml within 1.5 h without any additional procedure. 42 Hence, lectin sensitized gold nanoparticles were successfully applied for investigation of carbohydrate composition of cells. This approach has demonstrated that sensitized gold nanoparticles can be applied to study any ligand cell interaction with very high sensitivity.…”

Section: Colloidal Gold and Silver Preparationmentioning

confidence: 99%

Preparation and application of various nanoparticles in biology and medicine

Gasparyan

2013

Nanotechnol Dev

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The present paper considers prospects for application of various nanoparticles in biology and medicine. Here are presented data on preparation of gold and silver nanoparticles, and effects of shape of these nanoparticles on their optical properties. Application of these nanoparticles in diagnostics, for drug delivery and therapy, and preparation of magnetic nanoparticles from iron and cobalt salts are also discussed. Application of these nanoparticles as magnetic resonance imaging (MRI) contrast agents and as vehicles for drug delivery, and preparation of quantum dots and their application as prospective nanoparticles for multiplex analysis and for visualization of cellular processes will be tackled. Finally, prospects for new types of nanocomposites (metallic nano-shells) will be not overlooked

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“…Really, as a result of such agglutination process the major absorbance band is modified and a new absorbance band appears in long-wave region. Such immunoagglutination assays on nanoparticles of colloidal gold were widely applied for quantitative determination of various antigens [2][3][4][5] and for investigation of cell-ligand interactions [6]. In fact, this procedure provides very sensitive and specific one-step method for detection of any ligand-ligand interaction.…”

Section: Introductionmentioning

confidence: 99%