Silver sol immunoagglutination assay for determination of human IgG

Gasparyan, Vardan K.

doi:10.1016/j.colsurfb.2010.06.001

Cited by 13 publications

(12 citation statements)

References 16 publications

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“…4,22 The biological recognition element typically consists of naturally occurring recognition species such as antibodies, aptamers, and/ or DNA. 4 These molecules possess high selectivity and affinity for target biomolecules which lead to specic detection of biomarkers, 16,19 proteins, [6][7][8][9][10][11][12][13]15,[20][21][22][23]25,62 and viruses. 14,24 Improvement to optical biosensors can be made by incorporating noble metal nanomaterials for improved signal transduction allowing for numerous "enhanced" detection methods.…”

Section: Naturally Occurring Recognition Elements For Optical Sensorsmentioning

confidence: 99%

“…2 Typical assays times range anywhere from 2 to 60 minutes and additional pretreatment and washing steps which can take hours to days. 2 To further improve the signal to noise of optical affinity biosensor responses, noble metal nanomaterials are oen incorporated allowing for numerous "enhanced" detection methods such as such as colorimetry, [6][7][8][9][10] surface enhanced Raman spectroscopy (SERS), [11][12][13][14][15][16] and surface plasmon-based methods. [17][18][19][20][21][22][23][24][25] Biosensors which incorporate noble metal nanomaterials were extensively reviewed elsewhere and specically focused on localized and surface plasmon biosensors as well as SERS biosensors.…”

Section: Introductionmentioning

confidence: 99%

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Advancements in nanosensors using plastic antibodies

Volkert

Haes

2014

Analyst

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Biosensors possess recognition elements that bind to target molecules which lead to detectable signals. Incorporation of noble metal nanomaterials into biosensors allows for rapid and simple biomolecule detection. Herein, recent developments in affinity nanosensors will be discussed. These sensors often include naturally occurring recognition elements such as antibodies and DNA. As samples become more complex, new recognition elements are sought. For instance, plastic antibodies provide alternative and more environmentally stable recognition elements than traditional antibodies. Molecular imprinted polymers, a class of plastic antibodies, promote biomolecule recognition and detection. The incorporation of noble metal nanomaterials into molecular imprinted polymer biosensors for real world applications will be explored. Further improvements in the design of artificial recognition agents are envisioned to facilitate new methods for complex biological and chemical analyses.

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Section: Naturally Occurring Recognition Elements For Optical Sensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advancements in nanosensors using plastic antibodies

Volkert

Haes

2014

Analyst

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“…In contrary to gold nanoparticles there are only limited publications about preparation and optical properties of silver nanoparticles with various shapes and sizes. [26][27][28][29] Application of gold and silver nanoparticles…”

Section: Colloidal Gold and Silver Preparationmentioning

confidence: 99%

“…Moreover it was shown that silver nanoparticles can be prepared in much more concentrated form than gold nanoparticles. 29 It opens new horizons for application of silver nanoparticles in such assays. Recently, it was reported about synthesis of polycrystalline silver nanoparticles that exhibit both bright luminescence and a large enhancement effect on the Raman scattering signals of proximal molecules.…”

Section: Colloidal Gold and Silver Preparationmentioning

confidence: 99%

Preparation and application of various nanoparticles in biology and medicine

Gasparyan

2013

Nanotechnol Dev

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The present paper considers prospects for application of various nanoparticles in biology and medicine. Here are presented data on preparation of gold and silver nanoparticles, and effects of shape of these nanoparticles on their optical properties. Application of these nanoparticles in diagnostics, for drug delivery and therapy, and preparation of magnetic nanoparticles from iron and cobalt salts are also discussed. Application of these nanoparticles as magnetic resonance imaging (MRI) contrast agents and as vehicles for drug delivery, and preparation of quantum dots and their application as prospective nanoparticles for multiplex analysis and for visualization of cellular processes will be tackled. Finally, prospects for new types of nanocomposites (metallic nano-shells) will be not overlooked

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“…[26][27][28] However, silver nanoparticles (AgNPs) have higher molar attenuation coefficient, lower cost and lower tendency for unspecific aggregation than AuNPs. [29][30][31] hIgG is a class of immunoglobulin predominant in human serum that play an important role in humoral immunity in various types of cells. 32 The concentration of hIgG can be significantly altered in pathological conditions such as infections and immunodeficiencies.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles

2017

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A simple colorimetric immunoassay for quantification of human immunoglobulin G (hIgG) is herein described. The assay is based on the aggregation inhibition of silver nanoparticles (AgNP) functionalized with hIgG antibody (anti-hIgG) on the surface. The aggregation is measured in terms of attenuance values ratio at 400 and 530 nm (A400/A530). A linear response between A400/A530 and hIgG concentration is observed in the range 25 -200 ng mL -1 , and the detection limit is estimated as 11 ng mL -1 hIgG.

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Silver sol immunoagglutination assay for determination of human IgG

Cited by 13 publications

References 16 publications

Advancements in nanosensors using plastic antibodies

Advancements in nanosensors using plastic antibodies

Preparation and application of various nanoparticles in biology and medicine

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles

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