Wheat germ agglutinin and Lens culinaris agglutinin sensitized anisotropic silver nanoparticles in detection of bacteria: A simple photometric assay

Mikaelyan, Mariam V.; Poghosyan, Gayane G.; Hendrickson, Olga D.; Dzantiev, Boris B.; Gasparyan, Vardan K.

doi:10.1016/j.aca.2017.05.022

Cited by 21 publications

(6 citation statements)

References 37 publications

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“…WGA binds specifically to N-Acetyl-D-glucosamine (GlcNAc) and was shown to interact with sialic acid residues [ 53 ]. Due to its binding profile, WGA is widely used to label cell membranes and tissues in scientific imaging [ 54 , 55 ], and was shown to detect specific Gram-positive and Gram-negative bacteria [ 56 ]. It expresses antifungal activity [ 57 ] and interacts with immune cells in several ways, such as inhibiting T lymphocyte proliferation [ 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

Lectin from Triticum vulgaris (WGA) Inhibits Infection with SARS-CoV-2 and Its Variants of Concern Alpha and Beta

Auth

Fröba

Große

et al. 2021

IJMS

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Even in the face of global vaccination campaigns, there is still an urgent need for effective antivirals against SARS-CoV-2 and its rapidly spreading variants. Several natural compounds show potential as antiviral substances and have the advantages of broad availabilities and large therapeutic windows. Here, we report that lectin from Triticum vulgaris (Wheat Germ Agglutinin) displays antiviral activity against SARS-CoV-2 and its major Variants of Concern (VoC), Alpha and Beta. In Vero B4 cells, WGA potently inhibits SARS-CoV-2 infection with an IC50 of <10 ng/mL. WGA is effective upon preincubation with the virus or when added during infection. Pull-down assays demonstrate direct binding of WGA to SARS-CoV-2, further strengthening the hypothesis that inhibition of viral entry by neutralizing free virions might be the mode of action behind its antiviral effect. Furthermore, WGA exhibits antiviral activity against human coronavirus OC43, but not against other non-coronaviruses causing respiratory tract infections. Finally, WGA inhibits infection of the lung cell line Calu-3 with wild type and VoC viruses with comparable IC50 values. Altogether, our data indicate that topical administration of WGA might be effective for prophylaxis or treatment of SARS-CoV-2 infections.

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Section: Introductionmentioning

confidence: 99%

Lectin from Triticum vulgaris (WGA) Inhibits Infection with SARS-CoV-2 and Its Variants of Concern Alpha and Beta

Auth

Fröba

Große

et al. 2021

IJMS

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“…Schmitt et al reported that peptide coupling was significantly affected by change in pH, as an increase of pH from 7.4 to 9.5 enabled optimum coupling efficiency of cRGDfK peptides to PVDMA . Here, the effect of coupling buffers on WGA, HPA, and ConA lectins was investigated using buffers with pH values commonly used in the literature. ,− The selection of these three lectins and the pH values tested were based on differences in molecular weight, isoelectric point, number of azlactone-reactive lysine residues, and sugar specificity (Table S1). Whereas higher pH buffers favor nucleophilic addition with primary amino nucleophiles, the rate of azlactone hydrolysis will also increase and consequently compromise the reactive groups available.…”

Section: Resultsmentioning

confidence: 99%

Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution

et al. 2019

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Lectin-functional interfaces are useful for isolation of bacteria from solution because they are low-cost and allow nondestructive, reversible capture. This study provides a systematic investigation of physical and chemical surface parameters that influence bacteria capture over lectin-functionalized polymer interfaces and then applies these findings to construct surfaces with significantly enhanced bacteria capture. The designer block copolymer poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) was used as a lectin attachment layer, and lectin coupling into the polymer film through azlactone−lectin coupling reactions was first characterized. Here, experimental parameters including polymer areal chain density, lectin molecular weight, and lectin coupling buffer were systematically varied to identify parameters driving highest azlactone conversions and corresponding lectin surface densities. To introduce physical nanostructures into the attachment layer, nanopillar arrays (NPAs) of varied heights (300 and 2100 nm) were then used to provide an underlying surface template for the functional polymer layer. Capture of Escherichia coli on lectin−polymer surfaces coated over both flat and NPA surfaces was then investigated. For flat polymer interfaces, bacteria were detected on the surface after incubation at a solution concentration of 10 3 cfu/mL, and a corresponding detection limit of 1.7 × 10 3 cfu/mL was quantified. This detection limit was 1 order of magnitude lower than control lectin surfaces functionalized with standard, carbodiimide coupling chemistry. NPA surfaces containing 300 nm tall pillars further improved the detection limit to 2.1 × 10 2 cfu/mL, but also reduced the viability of captured cells. Finally, to investigate the impact of cell surface parameters on capture, we used Agrobacterium tumefaciens cells genetically modified to allow manipulation of exopolysaccharide adhesin production levels. Statistical analysis of surface capture levels revealed that lectin surface density was the primary factor driving capture, as opposed to exopolysaccharide adhesin expression. These findings emphasize the critical importance of the synthetic interface and the development of surfaces that combine high lectin densities with tailored physical features to drive high levels of capture. These insights will aid in design of biofunctional interfaces with physicochemical surface properties favorable for capture and isolation of bacteria cells from solutions.

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“…The surface of bacteria is rich in carbohydrate molecules, such as peptidoglycan and lipopolysaccharide, which makes it possible for lectins to be used as biorecognition molecules for bacterial separation and detection. The lectins that have been reported for the recognition of pathogens mainly include concanavalin A (Dechtrirat et al, 2014), wheat germ agglutinin (Mikaelyan et al, 2017), and Ricinus communis Agglutinin I (J. Gao et al, 2010).…”

Section: Lectinsmentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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Foodborne pathogens contamination is one of the main sources of food safety problems. Although the existing detection methods have been developed for a long time, the complexity of food samples is still the main factor affecting the detection time and sensitivity, and the rapid separation and enrichment of pathogens is still an objective to be studied. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered to be an effective tool for rapid separation and enrichment of foodborne pathogens in food. Therefore, this study comprehensively reviews the development of MNPs in the separation of foodborne pathogens over the past decade. First, various biorecognition reagents for identification of foodborne pathogens and their modifications on the surface of MNPs are introduced. Then, the factors affecting the separation of foodborne pathogens, including the size of MNPs, modification methods, separation strategies and separation forms are discussed. Finally, the application of MNPs in integrated detection methods is reviewed. Moreover, current challenges and prospects of MNPs for the analysis of foodborne pathogens are discussed. Further research should focus on the design of multifunctional MNPs, the processing of large‐scale samples, the simultaneous analysis of multiple targets, and the development of all‐in‐one small analytical device with separation and detection.

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Wheat germ agglutinin and Lens culinaris agglutinin sensitized anisotropic silver nanoparticles in detection of bacteria: A simple photometric assay

Cited by 21 publications

References 37 publications

Lectin from Triticum vulgaris (WGA) Inhibits Infection with SARS-CoV-2 and Its Variants of Concern Alpha and Beta

Lectin from Triticum vulgaris (WGA) Inhibits Infection with SARS-CoV-2 and Its Variants of Concern Alpha and Beta

Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

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