Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 °C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future.
Effective utilization of winter bamboo shoot shell (BSS) is of great interest, since BSS provides a renewable and inexpensive bioresource for the production of biofuels. In this study, an effective combination pretreatment by the sequential aqueous ammonia (25 wt%) extraction at 50 °C for 24 h and LiCl/N,N-dimethyl formamide (LiCl/DMF) (6 wt% of LiCl) pretreatment at 50 °C for 8 h was used for pretreating BSS. SEM, FTIR, and XRD results indicated that combination pretreatment could effectively remove lignin and change the crystal structure of cellulose for promoting enzymatic saccharification. Additionally, significant linear correlations were found about solid recovery-delignification (R = 0.9235), delignification-reducing sugars (R = 0.9552), and delignification-hemicellulose removal (R = 0.9779) during the combination pretreatment. The reducing sugars and glucose from the hydrolysis of 100 g/L pretreated BSS could be obtained at 72.3 and 40.5 g/L, respectively. Using the recovered BSS-hydrolysates containing 20-50 g/L glucose as carbon source, the ethanol yields at 48 h could be obtained at 84.5-86.1% of the theoretical yield. In conclusion, the sequential ammonia extraction and LiCl/DMF pretreatment has high potential application in future.
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