Enzymatic in situ saccharification of sugarcane bagasse pretreated with low loading of alkalic salts Na2SO3/Na3PO4 by autoclaving

Jiang, Chunxia; He, Yiming; Chong, Gang-Gang; Di, Junhua; Tang, Ya‐Jie; Ma, Cuiluan

doi:10.1016/j.jbiotec.2017.08.004

Cited by 34 publications

(11 citation statements)

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“…Bioethanol production from Kraft pulp by several fermentation configurations was studied, namely separate hydrolysis and fermentation (SHF) [22,23], simultaneous saccharification and fermentation (SSF) [15,[24][25][26][27], and consolidated bioprocessing [28].Besides hexose sugars, hydrolysates also have a high content in pentoses, mainly xylose, which can reach 25%, meaning that pentoses fermentation is necessary to attain an economically viable 2G bioethanol production [7,29]. Scheffersomyces stipitis was well as Saccharomyces cerevisiae have already been tested for bioethanol production from different LCB feedstocks, including eucalypt spent sulfite liquor [30][31][32], grape skins [33], sugarcane bagasse [34,35], cardoon, and rockrose [36]. The co-culture of hexose-and pentose-fermenting yeasts is a potential solution for this problem, since most well-known natural microorganisms are not able to efficiently ferment both sugars.…”

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confidence: 99%

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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Second-generation bioethanol production's main bottleneck is the need for a costly and technically difficult pretreatment due to the recalcitrance of lignocellulosic biomass (LCB). Chemical pulping can be considered as a LCB pretreatment since it removes lignin and targets hemicelluloses to some extent. Chemical pulps could be used to produce ethanol. The present study aimed to investigate the batch ethanol production from unbleached Kraft pulp of Eucalyptus globulus by separate hydrolysis and fermentation (SHF). Enzymatic hydrolysis of the pulp resulted in a glucose yield of 96.1 ± 3.6% and a xylose yield of 94.0 ± 7.1%. In an Erlenmeyer flask, fermentation of the hydrolysate using Saccharomyces cerevisiae showed better results than Scheffersomyces stipitis. At both the Erlenmeyer flask and bioreactor scale, co-cultures of S. cerevisiae and S. stipitis did not show significant improvements in the fermentation performance. The best result was provided by S. cerevisiae alone in a bioreactor, which fermented the Kraft pulp hydrolysate with an ethanol yield of 0.433 g·g −1 and a volumetric ethanol productivity of 0.733 g·L −1 ·h −1 , and a maximum ethanol concentration of 19.24 g·L −1 was attained. Bioethanol production using the SHF of unbleached Kraft pulp of E. globulus provides a high yield and productivity.Energies 2020, 13, 744 2 of 15 sustainability, has a low and stable price, and practically does not demand extra land [6,7]. There are some facilities producing 2G bioethanol on a commercial scale. However, large-scale production still faces some technological barriers that must be overcome in order to achieve a cost-competitive production [8]. Due to the recalcitrance of LCB, a costly pretreatment step is required, which is the main technological bottleneck of 2G bioethanol production. The release of enzymatic and fermentation inhibitors during pretreatment is another limitation [9].Pulp and paper mills have the infrastructures and logistics to handle LCB, and chemical mills employ technology required for LCB fractionation and conversion [10]. Bioethanol has been produced from different feedstocks such as Kraft pulp, spent sulfite liquor, and pulp and paper sludge [11]. Chemical pulping processes can be considered as a LCB pretreatment since they promote delignification and target hemicelluloses to some degree [12]. Chemical pulping represents about 77% of the virgin pulps produced globally, and more than 95% of these chemical pulps are Kraft pulps [13]. These pulps are produced by Kraft pulping involving the reaction of white liquor, i.e., an alkaline aqueous solution of sodium hydroxide and sodium sulfide with a pH of 14, with lignin at high temperature (150-170 • C). This reaction promotes lignin breakdown and degradation with the release of phenolic fragments, removing almost 90% of the lignin from the wood. Kraft pulping also leads to hemicelluloses and some cellulose loss and decreases the degree of polymerization of cellulose [14]. The utilization of Kraft pulping as a pretreatment method for LCB has ...

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Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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“…The released glucose concentration after hydrolysis HD was equivalent to the study of Wanderley et al (2013), in which the greatest glucose concentration (39 g/L) was achieved in the batch enzymatic hydrolysis of delignified SCB using 10 FPU/g cellulose of Celluclast 1.5 L and Trichoderma reesei β-glucosidase (Novozymes) at 50°C during 120 h. (de Andrade et al, 2017) performed a saccharification of 2% of alkaline pre-treated SCB with the enzymatic cocktail Multifect® CL supplemented with β-glucosidases from Chrysoporthe cubensis at similar conditions of enzymatic load, temperature, time and agitation and produced lower monosaccharide concentration (2.82 g/L of glucose and 0.98 g/L of xylose) than the present study. Jiang et al (2017) also obtained lower concentrations of TRS (33.8 g/L) and glucose (20.9 g/L) after enzymatic hydrolysis of Na2SO3/Na3PO4 pre-treated SCB with complexed enzymes (15 FPU/g and 60 CBU/g SCB). Comparing the achieved TRS, glucose and xylose yields to previous reports using the same conditions, it was concluded that the hydrolysis HD obtained significant quantities of released sugars and this hydrolysate was submitted to alcoholic fermentation as a fermentable sugar source.…”

Section: Enzymatic Hydrolysismentioning

confidence: 82%

“…(Wang et al, 2016) obtained a comparable ethanol concentration (17 g/L) after fermentation of hydrolysate of 5% PSB containing 61.0 g/L of glucose. Jiang et al (2017) fermented hydrolysate of Na2SO3/Na3PO4 pre-treated SCB with S. cerevisiae and achieved YP/S of 0.42 gethanol/gglucose.…”

Section: Ethanol Productionmentioning

confidence: 99%

Effects of the Increase in Substrate Load and Hydrolysis Time in the Saccharification of Sugarcane Bagasse and Ethanol Production

Lamounier

Pasquini

Rodrigues

et al. 2020

HOLOS

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“…The xylan, glucan, and lignin contents in reeds were measured according to the analytical performance of the National Renewable Energy Laboratory method . Soluble sugars in this liquid was measured with high-performance liquid chromatography (HPLC) equipped with a Bio-Rad Aminex HPX-87P column (Hercules, CA) and a refractive index detector (Waters 2414) to calculate the content of glucan and xylan. , The remaining solids by filtration were dried and weighed. It was placed in a crucible and fired in a muffle furnace at 550 °C for 4 h, and the mass before and after calcination was weighed, and the burned lignin content was calculated.…”

Section: Methodsmentioning

confidence: 99%

Sulfonated Vermiculite-Mediated Catalysis of Reed (Phragmites communis) into Furfural for Enhancing the Biosynthesis of 2-Furoic Acid with a Dehydrogenase Biocatalyst in a One-Pot Manner

Zhu

et al. 2020

Energy Fuels

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2-Furoic acid (FA), an upgrading product of furfural via oxidation, has been widely used in flavor, fragrance, polymer, agrochemical, and pharmaceutical industries, which can be manufactured from biomass-derived furfural. It is known that reed (Phragmites communis) is renewable, abundant, and inexpensive lignocellulosic biomass. In this study, FA was synthesized from reeds via the chemoenzymatic route. First, sulfonated tin-based vermiculite (Sn-vermiculite) was prepared as a solid acid catalyst to transform reeds into furfural, and 4.0 wt % loading of Sn-vermiculite gave furfural (55.0 mM) in a yield of 38.4% from NaOH-soaked reeds (AP-reeds) at 170 °C within 20 min. XRD, FT-IR, and SEM indicated that pretreated reeds had roughness and complexity of a wall surface because of severe pretreatments. In Sn-vermiculite-treated AP-reeds, over 98% of xylan in untreated reeds was removed. Furfural was obtained at 0.231 g furfural/g of xylan in AP-reeds. Furthermore, Escherichia coli HMFOMUT whole cells harboring dehydrogenase were used for biotransforming furfural to FA at 30 °C and pH 6.5. Finally, AP-reed-derived furfural was entirely into FA with 0.269 g FA/g of xylan in AP-reeds by E. coli HMFOMUT whole cells after 30 h. Significantly, this hybrid strategy was successfully established for transforming reeds to FA by sequential catalysis via the Sn-vermiculite catalyst and dehydrogenase biocatalyst.

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Enzymatic in situ saccharification of sugarcane bagasse pretreated with low loading of alkalic salts Na2SO3/Na3PO4 by autoclaving

Cited by 34 publications

References 44 publications

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Effects of the Increase in Substrate Load and Hydrolysis Time in the Saccharification of Sugarcane Bagasse and Ethanol Production

Sulfonated Vermiculite-Mediated Catalysis of Reed (Phragmites communis) into Furfural for Enhancing the Biosynthesis of 2-Furoic Acid with a Dehydrogenase Biocatalyst in a One-Pot Manner

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