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The IUPAC name of Cfx is (6R,7R)-7-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2(carboxymethoxyimino)acetyl] amino]-3-ethenyl-8-oxo-5-thia-1-azabicyc- ABSTRACTCefixime (Cfx) is a member of the third generation of Cephalosporin antibiotics. It used on a wide scale in prescribed antibiotic drugs as anti-infection for Gram-positive and Gram-negative microorganisms. The present study aimed to develop an HPLC method of Cfx analysis enjoyed highly linearity, repeatability, robustness, ruggedness, selectivity, rapidly, and economical to use. The chromatographic system depends on the RP-BDS column (250 mm x 4.6 mm x 5 μm). The mobile phase was prepared by mixing Methanol: Phosphate buffer (3:7, v/v) at flow rate 1.0 ml/min with wavelength detection at 254 nm, the temperature at 30° C with injection volume 20 μL. The method revealed that satisfied linearity regression R 2 (0.9996) with repeatability (0.94%) with DL and QL; 59.3 ng/ml and 179.8 ng/ml respectively. The method showed successful and satisfying results for Cfx in bulk and pharmaceutical formulations and urine samples at low levels.

Pharmaceutical wastewater contamination via azithromycin antibiotic and the continuous emergence of some strains of bacteria, cancer, and the Covid-19 virus. Azithromycin wastewater treatment using the biosynthesized Hematite nanoparticles (α-HNPs) and the biocompatible activities of the resulted nanosystem were reported. Biofabrication of α-HNPs using Echinaceapurpurea liquid extract as a previously reported approach was implemented. An evaluation of the adsorption technique via the biofabricated α-HNPs for the removal of the Azr drug contaminant from the pharmaceutical wastewater was conducted. Adsorption isotherm, kinetics, and thermodynamic parameters of the Azr on the α-HNPs surface have been investigated as a batch mode of equilibrium experiments. Antibacterial, anticancer, and antiviral activities were conducted as Azr@α-HNPs. The optimum conditions for the adsorption study were conducted as solution pH = 10, 150 mg dose of α-HNPs, and Azr concentration 400 mg/L at 293 K. The most fitted isothermal model was described according to the Langmuir model at adsorption capacity 114.05 mg/g in a pseudo-second-order kinetic mechanistic at R2 0.9999. Thermodynamic study manifested that the adsorption behavior is a spontaneous endothermic chemisorption process. Subsequently, studying the biocompatible applications of the Azr@α-HNPs. Azr@α-HNPs antibacterial activity revealed a synergistic effect in the case of Gram-positive more than Gram-negative bacteria. IC50 of Azr@α-HNPs cytotoxicity against MCF7, HepG2, and HCT116 cell lines was investigated and it was found to be 78.1, 81.7, and 93.4 µg/mL respectively. As the first investigation of the antiviral use of Azr@α-HNPs against SARS-CoV-2, it was achieved a safety therapeutic index equal to 25.4 revealing a promising antiviral activity. An admirable impact of the use of the biosynthesized α-HNPs and its removal nanosystem product Azr@α-HNPs was manifested and it may be used soon as a platform of the drug delivery nanosystem for the biomedical applications.

A new series of solid Cr(II), Co(II), Cu(II), Pd(II), and Cd(II) chelates of the 1-((E)-(2-mercaptophenylimino)methyl)naphthalen-2-ol (H 2 L) Schiff base ligand were synthеsized and chаracterized by diffеrent аnalytical and spectroscopic tеchniques. The results revealed the geometry around central metal ion to be tetrahedral for Cr 2 L 2 , Co 2 L 2 , Cu 2 L 2 , and Cd 2 L 2 complexes and square planar geometry around Pd 2 L 2 complex. 3D modeling of the ligand and its metal complexes was obtained using DFT/B3LYP method with LANL2DZ basis set. Also quantum-mechanical properties like molecular orbitals and molecular energies were calculated. The Cu nano complexes were prepared in different media Spinacia oleracea (SO), Corchorus olitorius (CO), Coriandrum sativum (CS), Eruca sativa (ES), and Anethum graveolens (AG) extract in EtOH. The particle size of the nano coordination Cu complexes depends on the high concentration of reducing agent present in the green leaf extract. The nature of the newly nano sizеd Cu complexes prepared in different media as crystalline or amorphous and particles size was determined by X-ray diffraction (XRD); scanning electron microscope (SEM). The Cu nano complexes are characterized by spectroscopic and thermal analysis techniques before heating and after heating upon 200 C. The Cu nano complexes were showed an antitumor activity towards hepatocellular carcinoma cell line (HepG-2). The antitumor data revealed that the Cu nano complex prepared in CS/EtOH (IC 50 $ 4.9 μg ml À1 ) has antitumor activity nearly to the activity of cis-platin (IC50 $ 1.143 μg ml À1 ). The DNA cleavage study showed that the ability for the screened Cu nano complex prepared in CS/EtOH to cleavage DNA. Therefore, it may be used as antitumor drugs in vivo to prevent the DNA replication in the tumor cells and to suppress the cancer for further increasing. Docking studies of the ligand and its Cu(II) complex showed good binding energy score ranging from (À4.73 to À5.98 kcal mol À1 ).

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