Molecular absorbance and fluorescence measurements are usually performed by benchtop or portable USB spectrophotometers or fluorometers. However, even the simplest configuration of these instruments requires considerable financial input, which is not affordable to many universities and research groups especially when public politics withdraw funds from science. Light-emitting diodes (LEDs) based devices can be an interesting alternative for low-cost, portable yet reliable measurements of absorbance of fluorescence. Especially if associated with the fabrication of 3D structures by additive manufacturing techniques. Although there are many reviews in the literature dedicated to discussing the theoretical aspects and analytical performance of LED-based devices, we consider that these reviews are not easily applicable for beginners in electronics. It results in the intimidation to employ such powerful tools which could have a huge impact mainly to small and/or initial groups. In this tutorial, we present a guide that will help students and researchers to construct “lab-built” devices based on simple optoelectronic components and 3D printing to perform molecular absorbance and fluorescence measurements for analytical methods. Finally, with this tutorial guide, we anticipate a large spread of the usage of LED devices for reliable analytical measurements that can be extensively used in many scenarios.
The application of urea-based selective catalytic reduction products (i.e., Urea-SCR) provides a reduction of NOx and, therefore, minimizes pollution emissions from vehicles fueled by diesel. Such products can be easily found in the market; however, they are often susceptible to adulteration, mainly in terms of the urea content and dilution with non-mineralized water. In this study, we propose a simple, low-cost, disposable, and straightforward paper-based microfluidic device for the quality-control of Urea-SCR products for the first time by quantifying urea and water hardness simultaneously via colorimetric reactions using a small volume of sample. 4-(dimethylamino)benzaldehyde and Eriochrome T were used as colorimetric indicators for urea and water hardness determination, respectively. Each reagent (1.5 µL) was combined with 6 µL of sample for analysis, contributing to an expressive reduction of waste generation. Digital images of the µPAD were obtained, and linear relations between color intensity and urea and Ca2+ and Mg2+ concentrations in the range of 0.2 to 1.0% and 0.1 to 3.5 mmol L−1 were obtained with a correlation coefficient higher than 0.99. Recovery experiments were employed to evaluate the accuracy of the methodology, revealing suitable values between 91.5 and 115%. Brazilian Urea-SCR samples were acquired from different distributors and submitted to the proposed procedure to evaluate its applicability. The application of microfluidic paper-based devices with colorimetric reactions enables the quality control of Urea-SCR products with high accuracy, portability, low consumption of reagents, and no generation of toxic residues; thereby contributing to the green analytical chemistry field.
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