The achievement of modern biotechnology, in particular genetic engineering in crop production, provides not only the possibility of increasing production, but also more efficient use of natural resources and reduces the negative impact on the environment. Recent publications indicate the widespread distribution of genetically modified (GM) agricultural crops in the world that are currently in commercial use, research and development. All over the world, the most cultivated GM crops are cotton, soybeans, corn, and rapeseed, and the area under these biotechnological crops continues to grow every year. However, the analysis of publicly available sources of information revealed the risks of negative effects of GMOs on human and animal bodies, as well as on the environment and biodiversity. The article analyzes the results of research on the presence of GM ingredients in plant raw materials, processing products, and feed for productive and non-productive animals for the period 2019-2021. The study was conducted by the polymerase chain reaction method in real time (PCR-RF), according to DSTU ISO 21569:2008 (ISO 21569:2005, IDT), and DSTU ISO 21571:2008 (ISO 21571:2005, IDT). In the studied samples were detected target sequences of the promoter p35S CaMV (cauliflower mosaic virus), promoter p34S FMV, terminator NOS (T-NOS) T1 of the Agrobacterium tumefaciens plasmid, genes Pat, EPSPS, Cry 3A. Transgenic plants are present in circulation in the agricultural market of Ukraine, and the area under their crops is increasing. The largest number of GM plants was found in samples of rapeseed, soybeans, and feed for productive and non-productive animals. There was a tendency for an increase in the number of transgenic plants in tested samples. During rapeseed research in 2019, the number of positive samples was 6.5%, in 2020 – 7.4%, and in 2021 – 14.3%. When studying soybeans in 2019, the number of positive samples was 6.7%, in 2020 – 16.7%, and in 2021 – 18.2%. GM ingredients were also found in feed for productive and non-productive animals. In 2020 16.7% of compound feed for poultry were positive, while in 2021 there were no samples for research. As for feeds for non-productive animals, during 2019–2020 there were no detected positive samples, while in 2021 the number of positive samples was 12%.
The aim of our work was to study the quality of vegetable oils - unrefined sunflower oil (grade I) and soy hydrated oil (grade I). One of the stages of the study was to determine the quality of vegetable oils - peroxide value (characterizes the amount of primary oxidation products of fats - peroxide compounds) and acid number (characterizes the total content of free fatty acids) in the samples of vegetable oils. The acid number was determined by titration (neutralization) of free fatty acids with alkali in the presence of an indicator (phenolphthalein). The peroxide number was determined by titration of the isolated iodine with a solution of sodium thiosulfate. Each study was reproduced 5 times. First of all, the quality indicators of oils (acid number and peroxide number) were determined in samples of fresh oils. Subsequently, the oil was kept for 3 months (in compliance with the recommended requirements for storage of oils and without compliance with the requirements for storage of oils) and re-determined these indicators. The quality of the investigated vegetable oils was determined by the indicators of acid number and peroxide number in accordance with the established requirements of the State Standards of Ukraine (DSTU EN ISO 660: 2009; DSTU 4570: 2006). The results of our research show that the level of acid number and peroxide level in samples of fresh oils meet the requirements of the State Standards of Ukraine (DSTU EN ISO 660: 2009; DSTU 4570: 2006) for this type of oil, which confirms the quality of oils and compliance with production , processing and transportation. It is shown that in the samples of aged oils (3 months, subject to storage requirements) the level of acid number and the level of peroxide number increases within normal limits. However, in the samples of aged oils (3 months, without compliance with storage requirements) there is a significant increase in the level of acid number and peroxide number, which does not meet the requirements of State Standards of Ukraine. As a result of research it is shown that the quality of vegetable oils depends on compliance with the recommended norms and conditions in the process of production, processing, transportation and storage. It is proved that under the conditions of violation of the recommended norms of storage in samples of high-quality vegetable oils the level of quality indicators of vegetable oils - acid number and peroxide number significantly increases.
The article highlights the need of the control of the quality of grain raw materials, because the increasing of the competitiveness of the grain industry is one of the leading in the national agrarian economy of Ukraine and largely its determines the place in the global agrarian market. In competition, only those enterprises retain and improve their position in the market, which consistently work to support and improve the quality of the products according to the important indicators for consumers. The main indicators of the grain quality are: protein content, and also humidity, smell, impurities, crude fat, ash content, etc. Todays, domestic enterprises introduce into the manufacturing the unique scientific and technical developments, which allow quickly and qualitatively conduct the research of raw materials and finished products. However, sometimes when controlling grain raw materials on such enterprises where the modern (spectrometric) research methods are used, some physico-chemical parameters do not coincide with the normative (arbitration) research methods. Therefore, in a comparative aspect were investigated samples of soybean, corn, barley and rapeseed by some qualitative indicators, such as humidity, crude protein and fat. By comparing two methods, it was found out that in the soybean and corn samples, both the arbitrage and the spectrometric methods were almost identical. In some rapeseed and barley samples, the difference between the same samples was small and was not more than 4.8 %. In determining the crude protein in the same samples in soybeans, the difference was from 0.9 to 5.1 %, corn grains – 2.6–5.6 %, barley grains – 0.9 to 5.8 %, rapeseed – 1.4–9.8 %. In determining the fat content of identical samples in soybeans, the difference was small and ranged from 3.8–6.2 %, corn grains 2.6–4.8 %, barley grains – 4.2–6.4 %, seeds rapeseed – 7.1–11.6 %. In the conducted interlaboratory researchers, in determining moisture, crude protein and fat in grain and oil raw materials, by both arbitration and spectrometric methods it was found out, that the results of the studies were within the statistically probable error, which indicate on the high efficiency the last one. In addition, the values of qualitative indicators of the researches samples responses the requirements of regulatory documents.
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