A simultaneous determination of three species by a flow injection analytical (FIA) method using enzyme-immobilized open-tubular reactors in parallel is described. The design of the optimal geometry of the FIA system is especially important to avoid overlap of any pairs of peaks. Ours was carried out based on the Taylor-Aris equation. Despite a somewhat complex splitting-confluence flow system, reproducible results were obtained in the simultaneous determination of L-lactate, f3-D-glucose and glycerol by the detection of the resulting common product, NADH. Assignment of the enzyme reactors to the channels should be made by taking into account the concentration ranges of the analytes to be measured and the differences in the rates of individual enzyme reactions.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.