Activity-directed fractionation of the inner stem bark of Tabebuia ochracea ssp. neochrysanta led to the isolation of two new naphtho[2,3-b]furan-4,9-diones, the 5,8-dihydroxy-2-(1′hydroxyethyl) and 2-acetyl-7-methoxy-8-hydroxy derivatives, 1 and 2, together with four known naphthofurandiones.The stem bark of Tabebuia ochracea ssp. neochrysanta (A. Gentry) A. Gentry (Bignoniaceae) has been used for many years by Tukuna indians of the Colombian Amazon as an antimalarial and for its healing action on ulcers. 1,2 This plant is native to tropical America, from El Salvador to northwest of Venezuela and Colombia and known in folk medicine as "To hua ri", "Vero", and "Cañ aguate". Zani et al. 3 isolated -sitosterol, cycloolivil, lapachol, and seven furanonaphthoquinones from the trunkwood of this plant. We found that the chloroform extract of T. ochracea ssp. neochrysanta showed cytotoxicity against melanoma B16 cells and antimalarial activity, in vitro, against strains of Plasmodium berghei. We report in this paper the structure elucidation of two new furanonaphthoquinones, 1 and 2, which were isolated along with the known 2-acetylnaphtho[2,3-b]furan-4,9-dione, 7-methoxy-8-hydroxy-2-(1′-hydroxyethyl)naphtho[2,3-b]furan-4,9-dione, 2-acetyl-7-methoxynaphtho[2,3-b]furan-4,9dione, and 7-methoxy-2-(1′-hydroxyethyl)naphtho[2,3b]furan-4,9-dione.