Aim. The present study was designed to investigate the effects of estradiol-ethylenediamine derivative on perfusion pressure and coronary resistance in rats. An additional aim was to identify the molecular mechanisms involved.Methods. The Langendorff model was used to measure perfusion pressure and coronary resistance changes in isolated rat heart after estradiol-ethylenediamine derivative alone and following compounds; tamoxifen (estrogen receptor antagonist), prazosin (alpha1 adrenoreceptor antagonist), metoprolol (selective beta1 receptor blocker), indomethacin (prostanglandin synthesis inhibitor) and nifedipine (L-type calcium-channel inhibitor).Results. The results show that estradiol-ethylenediamine derivative [10 -9 mmol] significantly increased perfusion pressure (p = 0.005) and coronary resistance (p = 0.006) in isolated rat heart. Additionally, the effect of estradiolethylenediamine on perfusion pressure [10 -9 to 10 -4 mmol] was only blocked in the presence of the L-type calciumchannel (nifedipine).Conclusions. These data suggest that the effect of estradiol-ethylenediamine on perfusion pressure and vascular coronary involves activation of the L-type calcium channel through a non-genomic molecular mechanism.
There are studies which show that several derivatives of brucine have been developed for its use in different biological and analytical methods 1-4. For example, a report shows the synthesis of N-chloromethylbrucine chloride by the reaction of brucine with dichloromethane 5. Other studies have shown the preparation of a brucine derivative (brucidine) by electrolytic reduction of brucine 6. In addition, there are reports of the synthesis of N-(5-carboxypentyl)brucinium bromide via N-alkylation of brucine with 6-bromohexanoic acid 7. Other experimental data showed the preparation of compounds brucinium hydrogen (S)-malate pentahydrate and anhydrous brucinium hydrogen (2R,3R)-tartrate by the reaction between brucine and D-L-malic acid or L-tartaric acid in ethanol:water 8. Additionally, the porphyrin-brucine conjugates was synthetized by the N-alkylation of brucine with alkylbromotetraphenylporphyrin derivatives 9. Recently, a brucine derivative (N 1-(2,3-dimethoxy strychnidin-10-yliden)-ethane-1,2-diamine) was synthetized by the reaction of brucine and ethylenediamine using boric acid as catalyst. Also other brucine derivative (11-[(2-aminoethylamino)-methyl]-2,3-dimethoxystrychnidin-10-ona) was prepared by the reaction of brucine with ethylenediamine in presence of formaldehyde 10. Additionally, other study showed the synthesis of a brucine-dihydropirymidine derivative using the multi-component system (brucine, benzaldehyde and
Isolated from oil-contaminated soil, Pseudomonas aeruginosa strain MGP-1 was identified by 16S rDNA sequence analysis and phenotypic characterisation. This strain was highly competent in medium and in soils, grew and survived well under adverse conditions. MGP-1 did not produce the pathogenic factor pyocyanin and used paraffin (C 11-40 ) as sole carbon source. It degraded eicosane (C 20 ) more efficiently than other n-alkanes, removing 60% within 16 days. An alkB gene and intermediate metabolites of mono-alcohol and lipid acid corresponding to the oxidised paraffin were detected in the cell-free extracts of MGP-1, indicating that it possessed a mono-terminal oxidation. This is the first report to demonstrate that a Pseudomonas strain is able to degrade paraffin with more than 18 carbon atoms by mono-terminal oxidation. Considering all the results, MGP-1 could be designed as a soil-born, highly competent, long chain n-alkane degrading bacterium with low or perhaps without pathogenicity.
As part of our screening of antioxidant extracts from spices and food plants, the antioxidant activity of the EtHO extract of the flowers of aerial parts of Sedum praealtum was tested. Since the extract showed significant activity, a bioassay-directed fractionation of it was carried out. This investigation resulted in the isolation of kaempferol and quercetin as the principle responsible for the antioxidant activity. The antioxidant ability was quantified using the Fogliano method, which is known as DMPD. Flavonols which were characterized by NMR. This similar antioxidant effect has been obtained on some fruits studied by different authors using this same method.
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