Cytoplasmic extracts of Vero cells infected with wild-strain Edmonston measles virus were found to contain two and probably three distinct species of nucleocapsids. Species sedimenting at 200 and 110S contained RNA which sedimented at 50 and 16 to 18S, respectively. The third nucleocapsid species which sedimented at 170S was not present in all experiments and was not characterized in detail. Essentially all 200 and 170S, as well as a portion of the 110S, nucleocapsids were membrane associated and probably present in part in cell-associated virions. Five of six plaque purified strains derived from wild-type Edmonston virus produced only 200S nucleocapsids. One of these five plaquepurified strains subsequently produced both 200 and 110S nucleocapsids after being passaged by using undiluted inocula. These results suggest that measles virus may produce distinct classes of defective virus containing short nucleocapsids and subgenomic viral RNA.
18 These procedures have been found to release 85% of the acid-precipitable DNA (labeled with tritiated thymidine) from adenovirus type 2 particles mixed with 108 nonradioactive KB cells.Furthermore, 90% or more of the acid-precipitable, tritiated thymidine-labeled material from adenovirus type 2 infected KB cells (exposed to tritiated thy ridine 12-24 hr after virus inoculation) and uninfected KB cells, similarly labeled, %as recoxered by these procedures. 19 Green, M., in Cold Spring Harbor Symposia on Quantitative Biology, vol. 27 (1962), p. 219. 20 As measured by the homology technique, there was no detectable adenovirus type 2 DNA synthesis (less than 1% of that in control cultures) in infected KB cells treated with 2 X 10-5 M FUDR. 21 Black, P. H., E. M. Crawford, and L.
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