Decreased Reactivity of SSPE Strains of Measles Virus with Antibody

Payne, Francis E.; Baublis, Joseph V.

doi:10.1093/infdis/127.5.505

Cited by 43 publications

(18 citation statements)

References 15 publications

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“…So far, no single stable property has been found which is solely a characteristic of measles virus isolates from CNS tissue, with the possible exception of reduced sensitivity to neutralization by immune sera (Payne & Baublis, 1973). Similar serological differences have been seen by a competition radioimmunoassay between measles and SSPE viruses which have been interpreted as the result of minor antigenic differences between these agents (Hall et al, 1978).…”

Section: Discussionmentioning

confidence: 93%

Antigenic Characterization of Measles and SSPE Virus Haemagglutinin by Monoclonal Antibodies

Meulen¹,

Löffler²,

Carter³

et al. 1981

Journal of General Virology

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SUMMARYHybrid cells secreting monoclonal antibodies directed against the haemagglutinin (H) protein of measles virus (Edmonston) were produced by fusion of mouse myeloma cells with spleen cells derived from immunized mice. Measles antibodies secreted by these cells were tested for their ability to react with measles virus in immunoprecipitation experiments and assays of binding, neutralization, haemagglutination inhibition and haemolysin inhibition. On this basis 21 out of 75 hybridomas could be defined and divided into five functional groups with different properties. However, when tested against other measles virus strains, including those isolated from subacute sclerosing panencephalitis (SSPE) patients, normalized radioimmunoassay (RIA) binding titres showed that the extent to which a given antibody bound could vary greatly with the virus strain examined. Moreover, the biological actions within a group were found to be very heterogeneous, even when high antibody binding titres were observed. These results suggest that different measles virus strains, which are not distinguishable by polyvalent sera, do in fact possess antigenic differences. Furthermore, the functional significance of a given virus epitope may vary from strain to strain. Hybridoma antibodies were also used to demonstrate the occurrence of antigenic changes within the H polypeptide of SSPE virus during the course of a non-productive, persistent infection in vitro.

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Section: Discussionmentioning

confidence: 93%

Antigenic Characterization of Measles and SSPE Virus Haemagglutinin by Monoclonal Antibodies

Meulen¹,

Löffler²,

Carter³

et al. 1981

Journal of General Virology

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show abstract

“…46 The peculiar incomplete vi¬ ral maturation occurring in SSPE brain, as described in this communi¬ cation, could be the expression of this genetic variant.47 Plasma cells are com¬ monly seen in the brain parenchyma, but up to now they have not been found to contain viral structures.…”

Section: Clinical Featuresmentioning

confidence: 86%

Subacute Sclerosing Panencephalitis

Dubois‐Dalcq¹

1974

Arch Neurol

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“…Since cells infected with defective SSPE virus do not release cell-free virus, productive SSPE virus, which is released at high titer as infectious virus, has been widely examined and found to have physical and biological characteristics indistinguishable from those of measles virus (3,10,14,15) except for decreased sensitivity to SSPE virus-neutralizing antibodies compared with measles virus (12).…”

Section: Discussionmentioning

confidence: 99%

Growth of Measles and Subacute Sclerosing Panencephalitis Viruses in Human Neural Cell Lines

Sakaguchi

Yoshikawa

Yamanouchi

1984

Microbiology and Immunology

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Growth of cell‐free subacute sclerosing panencephalitis (SSPE) virus was compared with that of measles virus in three human neural cell lines: neuroblastoma, oligodendroglioma, and glioblastoma. The Edmonston strain of measles virus replicated in these neural cells as efficiently as in Vero cells. In contrast, the growth of the Mantooth strain of SSPE virus was suppressed moderately in neuroblastoma cells and markedly in oligodendroglioma and glioblastoma cells in spite of the induction of apparent cytopathic effects in these cells. Virus adsorption, defective interfering particles, interferon, and temperature sensitivity were not responsible for this low yield of SSPE virus in neural cell lines. Synthesis of viral proteins of SSPE virus was slower than that of measles virus in oligodendroglioma and glioblastoma cells. These results suggest that the slow rate of synthesis of viral proteins may be relevant to the low yield of SSPE virus in neural cells.

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Decreased Reactivity of SSPE Strains of Measles Virus with Antibody

Cited by 43 publications

References 15 publications

Antigenic Characterization of Measles and SSPE Virus Haemagglutinin by Monoclonal Antibodies

Antigenic Characterization of Measles and SSPE Virus Haemagglutinin by Monoclonal Antibodies

Subacute Sclerosing Panencephalitis

Growth of Measles and Subacute Sclerosing Panencephalitis Viruses in Human Neural Cell Lines

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