Isolation of Measles Virus from Cell Cultures of Brain from a Patient with Subacute Sclerosing Panencephalitis

Payne, Francis E.; Baublis, Joseph V.; Itabashi, Hideo H.

doi:10.1056/nejm196909112811103

Cited by 334 publications

(123 citation statements)

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“…Seventeen were black and 3 white. Control sera were obtained from: a) 20 age-and sex-matched hospitalized patients with far-advanced tuberculosis; all were receiving INH, 11 were black (B), 8 white (W) and 1 Latin American (LA); antinuclear fluorescence tests were negative in all; b) 28 patients with rheumatoid arthritis (RA) (10 B, 16 W, 2 LA); c) 13 Antibody titers were determined in the laboratories of the Virology Section of the Center for Disease Control, US Public Health Service, Atlanta, Ga. The complementfixation microtiter technic (24) was used to determine antibody titers to the following myxovirus antigens: purified ribonucleoprotein (soluble antigen) of mumps virus and influenza virus Types A and B; and unpurified whole-virus preparations of influenza virus Type C, parainfluenza virus Types 1, 2 and 3, mumps virus, measles and respiratory syncytial virus.…”

Section: Methodsmentioning

confidence: 99%

Virus antibody levels in systemic lupus erythematosus

Hurd

Dowdle

Casey

et al. 1972

Arthritis & Rheumatism

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Antibody titers to a group of viral antigens have been determined in sera from patients with systemic lupus erythernatosus (SLE), control groups with inflammatory diseases and normals. Mean titers in SLE sera for all viruses tested were significantly greater than in four control groups, but not greater than in active tuberculosis, both by the complement-fixation (CF) and hemagglutination-inhibition (HI) methods. By the CF method, only measles virus showed significantly higher titers in SLE than in all control groups; by the HI method, measles antibody titers were higher in SLE than in all groups but tuberculosis. There was no correlation between antibody titers and gammaglobulin levels. The results indicated a moderate though variable overall hypereactivity in SLE to the viral antigens tested.Interest has grown recently in the possibility that the etiology of SLE is viral in nature. One reason for this interest has been the observation of cytoplasmic myxovirus-like tubular structures in the tissues of patients with SLE. These have been most commonly reported (1-5) in kidney biopsies of patients with lupus nephritis. The cytoplasmic structures described are simi-

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Section: Methodsmentioning

confidence: 99%

Virus antibody levels in systemic lupus erythematosus

Hurd

Dowdle

Casey

et al. 1972

Arthritis & Rheumatism

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Section: Discussionmentioning

confidence: 99%

“…Several measles-like viruses (strains?) have been isolated by laboratory co-cultivation techniques, from cases of subacute sclerosing panencephalitis (SSPE) (Horta-Barbosa et al, 1969;Payne et al, 1969).During an investigation of the growth properties of an MV SSPE isolate, designated Halle (MVH), a temperature-dependent plaquing phenomenon (termed ring plaques) was observed. A study of the ring plaque growth phenomenon led to the isolation of MV interfering particles.…”

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confidence: 99%

An Interference Phenomenon Associated with a Measles Virus SSPE Isolate (Halle)

Tsang

Chang

Marusyk

1981

Journal of General Virology

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SUMMARYA measles virus (Halle SSPE isolate) induced ring plaque phenomenon (concentric rings of living and dead cells) has been shown to be associated with the temperaturedependent production of interfering particles. The interfering particles have been purified by potassium tartrate linear gradient centrifugation and have buoyant densities of 1.15 g/ml (M particle) and 1-06 g/ml (L particle) respectively. Both interfering particle populations have been shown to decrease the yield of wild-type measles virus from infected cells by 50% when co-infection experiments were performed. Neither the M or L particle population interfered with the growth of VSV in the same host-cell system.Measles virus (MV), a member of the Paramyxoviridae family (genus Morbillivirus) is currently undergoing extensive investigation, particularly with regard to its possible involvement in the aetiology of certain neurological diseases and/or disorders (ter Meulen & Hall, 1978). Several measles-like viruses (strains?) have been isolated by laboratory co-cultivation techniques, from cases of subacute sclerosing panencephalitis (SSPE) (Horta-Barbosa et al., 1969;Payne et al., 1969).During an investigation of the growth properties of an MV SSPE isolate, designated Halle (MVH), a temperature-dependent plaquing phenomenon (termed ring plaques) was observed. A study of the ring plaque growth phenomenon led to the isolation of MV interfering particles. This communication describes and discusses the possible implications of the phenomenon observed.The MVH isolate (obtained from Dr T. F. Wild, INSERM, Lyon, France) was twice plaque-purified in cultures of Vero cells (ATCC CCL81; Flow Laboratories). As many laboratories have noted that certain strains of MV grow to higher titres at 32 °C than at 37 °C (T. F. Wild, personal communication; A. Salmi, personal communication; this study), the MVH isolate was grown at 32 °C, 37 °C and 39 °C and examined at regular intervals for plaque production. At 5 days post-infection, the virus grown at 37 °C showed plaques with clear centres surrounded by a ring of cells (Fig. 1). As incubation progressed (up to 20 days post-infection) more rings appeared as the plaques enlarged until a concentric ring pattern appeared (ring plaques). Vital dye (neutral red) staining indicated that the ring plaques consisted of alternating, concentric rings of living and dead cells. Plaques observed at 32 °C and 39 °C were distinct from those seen at 37 °C (Fig. i) and at no time were the characteristic ring plaques seen at 32 °C or 39 °C. Use of alternate host-cell systems for plaque titration of the MVH isolate (BSC-1, ATCC CCL26; HEp-2, ATCC CCL23; KB, ATCC CCL17) gave similar results, although the ring plaques produced were considerably smaller in size (not shown). Such results would indicate that the phenomenon is not host-cell dependent. The larger plaque size observed in the Vero host-cell system can be related to the greater efficiency of MV replication in that particular cell system. Plaque titration of the MV LEC SSPE isolat...

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“…MV can cause four distinct syndromes in humans: the classical epidemic acute illness of children, an autoimmune-like condition known as post-infectious encephalomyelitis (Norrby & Oxman, 1990;Kipps et al, 1983), late and usually fatal complications of acute measles infection known as subacute sclerosing panencephalitis (SSPE) and measles inclusion body encephalitis (MIBE). These complications develop as a result of the persistence of measles virus in brain cells (Baczko et al, 1984;Basle et al, 1987;Carter et al, 1983;Cattaneo et al, 1987Cattaneo et al, , 1988Kipps et al, I" Present address: Department of Molecular Pharmacology, Albert Einstein School of Medicine of Yeshiva University, 1300 Morns Park Avenue, Bronx, New York, New York 10461, U.S. Payne et al, 1969). MV replication in the brains of SSPE and MIBE patients is characterized by defective expression of the viral envelope proteins (Baczko et al, 1984Cattaneo et al, 1988).…”

Section: Introductionmentioning

confidence: 99%