This experiment aims to determine the correlation of total phenolic and flavonoid content of jati putih leaves fraction (Gmelina arborea Roxb.) towards Antioxidant activity . Sample was extracted by maceration method using ethanol 70% to obtain the ethanol extract (EE), followed by liquid-liquid extraction method to obtain fraction of ethyl acetate (EA) and n-Hexane (EH). The phytochemical screening and determination of total phenolic and flavonoid content were done by colorimetric method. Antioxidant activity were done by DPPH, FRAP and ABTS methods. Phytochemical screening showed positive results for flavonoids, phenolic and saponins. The largest total phenolic content was found on EA (11,59 µg/ml ± 0,3 %b/b EAG) and the largest total flavonoid content was on EA (3,88 µg/ml ± 0,02 %b/b EK). The total phenolic and flavonoid content of Jati putih leaves has a correlation with antioxidant activity. The coefficient correlation of activity on reducingDPPH radical was 56,7% (total of phenolic content) and 57,8% (total of flavonoid content) and on iron reduction power in FRAP method was 99,9% (total of phenolics and flavonoids content). The relationship with the activity in reducing radical ABTS obtained coefficient correlation of 57,0% and 58,1% for total phenolic and flavonoids contents, respectively.
Seaweed is one of the marine algae that have antioxidant sources because it contains
bioactive compounds such as carotenoids, phenol compounds and their derivatives,
polysaccharide sulfate, and vitamins. Algae consist of three major groups namely brown
algae (phaeophyta), red algae (rhodophyta), and green algae (chlorophyta). The purpose of
this study was to determine the bioactivity profile of seaweed extract Eucheuma cottoni,
Sargassum polycystum and Caulerpa racemosa so that it could be developed as a cosmetic
raw material. The three types of seaweed were extracted by maceration and bioactivity
testing was carried out as an antioxidant using the ABTS radical reduction method and
bioactivity testing as UV-protection with the parameters of the percentage transmission of
erythema, pigmentation and Sun Protective Factor (SPF). The results showed that C.
racemosa extract provided a very strong antioxidant activity and was able to protect the
skin from UV exposure with an SPF value that was categorized as ultra-protection.
Antioxidant activity of the three types of seaweed is positively correlated to the protective
effect based on the SPF value.
Telah dilakukan penelitian uji aktivitas antioksidan ekstrak metanol bunga brokoli (Brassica oleracea L. var. Italica) dengan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan metode ABTS (2,2-Azinobis (3etilbenzotiazolin)-6-asam sulfonat). Bunga brokoli diekstraksi secara maserasi dengan menggunakan pelarut metanol. Ekstrak bunga brokoli (Brassica oleracea L. var. Italica) dibuat dalam berbagai konsentrasi dan diuji aktivitas antioksidannya. Pada metode DPPH diperoleh nilai IC50 sebesar 123,698 ppm dan IC50 vitamin C murni sebesar 3 ppm sedangkan pada metode ABTS diperoleh nilai IC50 sebesar 32,1292 ppm dan nilai IC50 vitamin C murni sebesar 6 ppm. Kata Kunci : Antioksidan, Bunga Brokoli (Brassica oleracea L. var. Italica), metode DPPH dan Metode ABTS
This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL.
Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically
as an anticancer and antidote materials by Dayaknese in West Kutai, with limited
scientific study. Thus, this research had been conducted to determine and evaluate the total
phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile.
The research used several extracts’ solvents including extraction with ethanol (crude
extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and
ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed
through colorimetric method to determine the phenolic and flavonoid total; with DPPH
and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the
toxicity activity. The results showed that the EF fraction provided the strongest antioxidant
activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of
387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and
the total phenolic content (r2
= 0.970 - 0.974) but little correlation in total flavonoid (r2
=
0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity
followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the
paku atai tuber can be potentially developed as a natural antioxidant and anticancer
material.
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