UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (2,2 diphenyl-1-picrylhydrazyl) dan METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat)

Sami, Fitriyanti Jumaetri; Rahimah, Sitti

doi:10.33096/jffi.v2i2.179

Cited by 15 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another important consideration is that the bioactive compounds (flavonoids and phenolics) present in the corn kernels are in their glycoside form, which has been reported to have weak free radical scavenging activity (Molyneux 2004). Furthermore, the purity of the extract can also affect the antioxidant capacity since the presence of other compounds can weaken the free radical scavenging activity, as previously reported (Sami and Rahimah 2016). In conclusion, Based on the result and discussion, it can be concluded that the Pulut cultivar exhibited the highest total polyphenol content, while the Kuning Dalam cultivar reported the third highest total polyphenol content, the highest total flavonoid content and one of the strongest antioxidant activity.…”

Section: Pearson Correlation Coefficientssupporting

confidence: 67%

Determination of free radical scavenging activity, phenolic and flavonoid content of seven corn cultivars from the Southwest Maluku District, Indonesia

2022

View full text Add to dashboard Cite

Abstract. Sinay H, Mahulette F, Yáñez JA. 2022. Determination of free radical scavenging activity, phenolic and flavonoid content of seven corn cultivars from the Southwest Maluku District, Indonesia. Biodiversitas 23: 5974-5981. The variation of corn (Zea mays L.) kernel might be considered as a surrogate indicator of the phytochemical and bioactive content. This study aimed to determine the phenolics, flavonoids, and free radical scavenging activity of seven local corn cultivars from Kisar Island, Southwest Maluku District in Indonesia. Sample extraction was done by the maceration method. Flavonoid content was determined using the Dowd method, and total phenolic content was performed using the Folin-Ciocalteu method. Free radical scavenging activity was determined using the DPPH radical scavenging method. One-way ANOVA was performed with Duncan multiple range tests to assess the differences between corn seed cultivars, and p < 0.05 were regarded as statistically significant. The results show that the total flavonoid content varied from 8.463 mg QE/mg (Putih cultivar) to 40.075 mg QE/mg (Kuning Dalam cultivar). Total polyphenol content also varied between 105.63 mg GAE/mg (Merah Delima Tongkol Putih cultivar) and 136.41 mg GAE/mg (Pulut cultivar). The antioxidant activity by DPPH assay determined that the seven local corn cultivars had IC50 values ??that were similar to quercetin (used as control), some were even lower. The IC50 value of the seven local corn cultivars ranged from 5.56 to 16.96 µg/mL, while quercetin had an IC50 value of 11.97 µg/mL. It was observed that the Merah Delima Tongkol Cokelat, Merah Delima Tongkol Putih, and Putih cultivars had comparable antioxidant activity to the control quercetin. The Merah Darah, Kuning Genjah, and Kuning Dalam cultivars exhibited a statistically significant higher antioxidant capacity than quercetin. These results can contribute to the selection of corn cultivars to harvest for commercial purposes and potential nutraceutical applications.

show abstract

Section: Pearson Correlation Coefficientssupporting

confidence: 67%

Determination of free radical scavenging activity, phenolic and flavonoid content of seven corn cultivars from the Southwest Maluku District, Indonesia

2022

View full text Add to dashboard Cite

show abstract

“…The yield of evaporation was calculated (20). Then each extract was analyzed for IC-50 antioxidant activity (21,22). Data analysis was carried out descriptively (mean ± standard deviation).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity From Multiple Extraction Of Kratom Leaf (Mitragyna speciosa) Without Veins With Sonicator-Type Bath

Purwayantie¹,

Fadly²,

Sholahuddin³

et al. 2022

JHNR

View full text Add to dashboard Cite

Kratom is one of the export commodities from West Kalimantan, containing alkaloids that are beneficial to health. The export product currently in demand is extracted flour. So far, the most widely used extraction method is conventional single extraction, using maceration which is inefficient, long and consumes a lot of solvents. This study examined the process of alkaloid extraction using the technology of drying with a microwave and extraction using a sonicator. The study aimed to determine the microwave drying method which produces kratom leaf alkaloid levels and the type of solvent which produces the strongest IC-50 antioxidant properties from the bath-type sonicator multilevel extraction method. The research method was divided into 2 stages, namely, stage 1 drying with 2 ingredients, kratom leaves with veins and without veins, then tested for alkaloid levels. The leaves material with high alkaloids was continued for phase II of the IC-50 antioxidant property test from various solvents (hexane, methanol, ethanol) using multilevel extraction with a bath-type sonicator. Data analysis was carried out descriptively. The results showed that leaves without veins produced relatively higher levels of alkaloids than leaves with veins. The type of burner that produced IC-50 antioxidant activity was 0.3 ppm ethanol extract. Thus, these results can be a recommendation to stakeholders

show abstract

“…Antioxidant activity was analysed following the method used by Sami and Rahimah (2016). A total of 3 g leaf sample was macerated using methanol (99.8%) for 24 h. After 24 h, the mixture was filtrated using filter paper, and the filtrate was thickened utilising a rotary evaporator.…”

Section: Antioxidant Activity Analysismentioning

confidence: 99%

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Mubarok

Jadid²,

Widiastuti³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Fruit set is one of the main problems that arise in tomato plants under heat-stress conditions, which disrupt pollen development, resulting in decreased pollen fertility. Parthenocarpic tomatoes can be used to increase plant productivity during failure of the fertilisation process under heat-stress conditions. The aim of this study were to identify the plant adaptability and fruiting capability of ?iaa9-3 and iaa9-5 tomato mutants under heat-stress conditions. The iaa9-3 and iaa9-5 and wild-type Micro-Tom (WT-MT) plants were cultivated under two temperature conditions: normal and heat-stress conditions during plant growth. The results showed that under the heat-stress condition, iaa9-3 and iaa9-5 showed delayed flowering time, increased number of flowers, and increased fruit set and produced normal-sized fruit. However, WT-MT cannot produce fruits under heat stress. The mutants can grow under heat-stress conditions, as indicated by the lower electrolyte leakage and H2O2 concentration and higher antioxidant activities compared with WT-MT under heat-stress conditions. These results suggest that iaa9-3 and iaa9-5 can be valuable genetic resources for the development of tomatoes in high-temperature environmental conditions.

show abstract

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (2,2 diphenyl-1-picrylhydrazyl) dan METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat)

Cited by 15 publications

References 0 publications

Determination of free radical scavenging activity, phenolic and flavonoid content of seven corn cultivars from the Southwest Maluku District, Indonesia

Determination of free radical scavenging activity, phenolic and flavonoid content of seven corn cultivars from the Southwest Maluku District, Indonesia

Antioxidant Activity From Multiple Extraction Of Kratom Leaf (Mitragyna speciosa) Without Veins With Sonicator-Type Bath

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Contact Info

Product

Resources

About