Seaweed is one of the main commodity which spread throughout Indonesian waters. Seaweed contains bioactive compounds which can serve as a defense form ultraviolet radiation. The purpose of this study was to obtain chemical composition, phytochemical compounds, vitamin E, and antioxidant activity of extract P. australis and E. cottonii. Chemical composition of P. australis moisture 87.25%; ash 2.34%; protein 1.05%, fat 0.58%; and carbohydrates 8.78% while E. cottonii moisture 76.15%; ash 5.62%; protein 2.32%; fat 0.11%; and carbohydrates 15.8%. The yield of P. australis extracts using methanol 4.55%; ethyl acetate 0.8%; and n-hexane 0.45%, yield of E. cottonii extracts using methanol 6.6%; ethyl acetate 0.5%; and and n-hexane 0.35%. Vitamin E value of P. australis 162.75 μg/mL and E. cottonii 158.07 μg/mL. IC50 value of P. australis 87.082 ppm and E. cottonii 106.021 ppm. P. australis and E. cottonii contain phytochemical compound such as flavonoids, phenol hydroquinone, and triterpenoids, P. australis was also known contain tannins and<br />saponins.
Exposure of UV light can cause skin rashes and sunburn, stimulating the formation of melanin. Long term UV exposure also can cause skin cancer. One way that can reduce negative impact of UV exposure is by using sunscreen. The aims of this study were to obtained the best ratio of seaweed P. australis and E. cottonii slurry to produce sunscreen cream and as well as obtaining sunscreen formulation with good stability. Creams were formulated consist of cream with different ratio of seaweed P. autralis and E. cottonii slurry, i.e. A (control), B (1:1), C (2:1) and D (1:2). The commercial creams product also used as a comparator (KI and KII). The best ratio of P. australis and E. cottonii slurry was showed on cream B (1:1). Sunscreen creams have a good level stability, with no phase separation of dispersant and dispersed phase, and also no discoloration. Sunscreen creams have at least 1 year shelf life as there was no phase separation after centrifugal force at 3,800 rpm for 5 hours.
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